Screening of Lipolytic Bacteria from Cemetery Soil
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Submitted
5 September 2023
5 September 2023
Published
31 December 2023
31 December 2023
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Abstract
Background: The cemetery is an area for the burial of corpses so that active decomposition of bodies occurs. One of the decomposition bacteria that might be found is lipolytic bacteria because the human body comprises 21,33-32,51 % lipids. Explorations of lipolytic bacteria from cemeteries in Indonesia have not been found, so this research needs to be done. This non-experimental study aimed to select and identify lipolytic bacterial isolates from the Pracimaloyo cemetery, Surakarta. Method: A total of 36 bacterial isolates were selected for their lipolytic activity using tributyrin media. The lipolytic index determines lipolytic activity. Identification of lipolytic bacteria based on colonial morphology and Gram staining. Result: The study showed that 22.2% (8 isolates) showed a lipolytic positive, with the largest lipolytic index (LI) value of 2.5 (isolate P36). Bacterial colonies are circular-shaped, have entire edges, flat elevation, and are yellow or white. Gram staining results showed that the isolated is a group of Gram-negative bacteria in the form of coccus. Conclusion: The conclusion of this study suggests that bacterial isolates from Pracimaloyo cemetery that have potential lipolytic activity, which is likely to be from the genera Pseudomonas and Klebsiella, were obtained.
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References
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Dinçer, E., & Kıvanç, M. (2018). Lipolytic Activity of Lactic Acid Bacteria Isolated from Turkish Pastırma. Anadolu University Journal of Science and Technology C- Life Sciences and Biotechnology, 7(1), 12–19. https://doi.org/10.18036/aubtdc.306292
Dippenaar, M. A. (2014). Towards a Multi-Faceted Vadose Zone Assessment Protocol: Cemetery Guidelines and Application to a Burial Site Located Near a Seasonal Wetland (Pretoria, South Africa). Bulletin of Engineering Geology and the Environment, 73(4), 1105–1115. https://doi.org/10.1007/s10064-014-0635-3
Ethica, S. N. (2018). Buku Referensi Bioremediasi Limbah Biomedik Cair. Yogyakarta: Deepublish.
Evans, P. R., Whitchurch, D. M., & Muhlestein, K. (2015). Rethinking Burial Dates at a Graeco-Roman Cemetery: Fag el-Gamous, Fayoum, Egypt. Journal of Archaeological Science: Reports, 2, 209–214. https://doi.org/10.1016/j.jasrep.2015.02.004
Firdausi, W. & Zulaika, E. (2015). Potensi Azotobacter spp. sebagai Pendegradasi Lipid dan Protein. Bioeksperimen. Volume 1 No. 2. 18-21. https://doi.org/10.23917/bioeksperimen.v1i2.873.
Forbes, R. M., Cooper, A. R., & Mitchell, H. H. (1953). The Composition of the Adult Human Body as Determined by Chemical Analysis. The Journal of Biological Chemistry, 203(1), 359–366. https://doi.org/10.1016/s0021-9258(19)52646-1
Jamil, S. N. A., Wijaya, A., Sendra, E., Rahman, I. W., Chairiyah, R., Ulimaz, A., Lindawati. (2022). Mikrobiologi. Padang: Global Eksekutif Teknologi Press.
Kawuri, R., & Darmayasa, I. B. G. (2022). Potensi Bakteri Sebagai Biodegradasi Lemak dan Minyak pada Lingkungan Yang Tercemar Limbah Domestik. Metamorfosa: Journal of Biological Sciences, 9(1), 184. https://doi.org/10.24843/metamorfosa.2022.v09.i01.p18
Kaysi, A. F. El. (2018). Panduan Praktis Shalat Jenazah & Perawatan Jenazah. Yogyakarta: Media Pressindo.
Khairani, & Manalu, K. (2023). Isolasi dan Identifikasi Bakteri Lipolitik dari Limbah Cair Kelapa Sawit (Elaeis quineensis Jacq.). BIOEDUSAINS: Jurnal Pendidikan Biologi Dan Sains, 6(1), 1–13. https://doi.org/10.31539/bioedusains.v6i1.5285
Lanka, S., & Latha, J. N. L. (2015). A Short Review on Various Screening Methods to Isolate Potential Lipase Producers: Lipases-the Present and Future Enzymes of Biotech Industry. International Journal of Biological Chemistry, 9(5), 207–219. https://doi.org/10.3923/ijbc.2015.207.219
Mazhar, H., Abbas, N., Zamir, T., Hussain, Z., & Ali, S. S. (2018). Optimization Study of Lipolytic Enzyme from Bacillus cereus, PCSIR NL-37. Punjab University Journal of Zoology, 33(2), 217–224. https://doi.org/10.17582/journal.pujz/2018.33.2.217.224
Nasikhin, R., & Shovitri, M. (2013). Isolasi dan Karakterisasi Bakteri Pendegradasi Solar dan Bensin dari Perairan Pelabuhan Gresik. Jurnal Sains Dan Seni Pomits, 2(2), 84–88. http://dx.doi.org/10.12962/j23373520.v2i2.3626
Oktavia, D. A., & Wibowo, S. (2017). Penapisan dan Identifikasi Bakteri Lipolitik yang Diisolasi dari Air Limbah Pengolahan Surimi dan Pengalengan Rajungan. Jurnal Pascapanen Dan Bioteknologi Kelautan Dan Perikanan, 11(2), 147. https://doi.org/10.15578/jpbkp.v11i2.262
Pemerintah, D. Penyelenggaraan dan Pelayanan Pemakaman (2013). Magelang.
Putra, S. S., Rahayu, T., & Tyastuti, E. M. (2023). Isolation and Characterization of Cambodian Tree Rhizospheric Bacteria (Plumeria acuminata) at Pracimaloyo TPU as a producer of IAA. BIOEDUSCIENCE, 7(1), 15–23. https://doi.org/ 10.22236/jbes/7111375
Remijawa, E. S., Rupidara, A. D. N., Ngginak, J., & Radjasa, O. K. (2020). Isolasi dan Selesksi Bakteri Penghasil Enzim Ekstraseluler pada Tanah Mangrove di Pantai Noelbaki. Jurnal Enggano, 5(2), 164–180. https://doi.org/10.31186/jenggano.5.2.164-180
Rizky, M. Y., Fitri, R. D., Hastuti, U. S., & Prabaningtyas, S. (2017). Identifikasi Uji Kemampuan Hidrolisis Lemak dan Penentuan Indeks Zona Bening Asam Laktat pada Bakteri dalam Wadi Makanan Traditional Kalimantan Tengah. Bionature, 18(2), 87–98. https://doi.org/10.35580/bionature.v18i2.6137
Sayuti, I., & Suratni. (2015). Isolasi dan Identifikasi Bakteri Hidrokarbonoklastik dari Limbah Cair Minyak Bumi GS Cevron Pasifik Indonesia di Desa Benar Kecamatan Rimba Melintang Rokan Hilir. Prosiding Semirata, 4(1), 320–334.
Siregar, R. N. F., Suwandi, J. F., & Kurniati, I. (2022). Entomologi Forensik. Agromedicine, 9(1), 76–80.
Sumarlin, L. O., Mulyadi, D., Suryatna, & Asmara, Y. (2013). Identifikasi Potensi Enzim Lipase dan Selulase pada Sampah Kulit Buah Hasil Fermentasi. Jurnal Ilmu Pertanian Indonesia (JIPI), 18(3), 159–166.
Suyanto, E., Soetarto, E. S., & Cahyanto, M. N. (2015). Produk Lipase Kapang Lipolitik pada Limbah Ampas Kelapa. Bioeksperimen: Jurnal Penelitian Biologi, 1(1), 12–17. https://doi.org/10.23917/bioeksperimen.v1i1.311
Syah, M. A. (2022). Isolasi dan Karakterisasi Molekuler Gen 16S rRNA Bakteri Lipolitik Asal Limbah Kulit Biji Jambu Mete. Sumberdaya Hayati, 8(1), 20–26. https://doi.org/10.29244/jsdh.8.1.20-26
Tsani, F. F. (2021). Isolasi dan Karakteristik Bakteri Lipolitik dari Tanah Tempat Pemrosesan Akhir (TPA) Talangagung Kepanjen Kabupaten Malang. Universitas Islam Negeri Maulana Malik Ibrahim Malang.
Turajo, K. A., Abubakar, B. S. U. I., Dammo, M. N., & Sangodoyin, A. Y. (2019). Burial Practice and its Effect on Groundwater Pollution in Maiduguri, Nigeria. Environmental Science and Pollution Research, 26(23), 23372–23385. https://doi.org/10.1007/s11356-019-05572-6
Upayogi, I. N. T. (2019). Kajian Eksplanasi Taru Menyan Penetral Bau Mayat. Jurnal Filsafat Indonesia, 2(1), 37. https://doi.org/10.23887/jfi.v2i1.17550
Widiastuti, L., Sulistiyanto, Y., Jaya, A., Jagau, Y., & Neneng, L. (2019). Potensi Mikroorganisme Sebagai Biofertilizer Dari Limbah Cair Pabrik Kelapa Sawit. Jurnal Surya Medika, 5(1), 1–12. https://doi.org/10.33084/jsm.v5i1.941
Yousef, A. E., Cusic, J. G. W., & Perry, J. J. (2022). Analytical Food Microbiology: A Laboratory Manual. Amerika Serikat: Wiley.
Zainuddin, Habib, Z., Rachmadhani, A., Nuriyanto, K., Wibowo, A. M., & Solikin, N. (2022). The Impact Of State Regulations On Social Changes Futures As Cultural Spaces. Journal of Positive School Psychology, 6(6), 4281–4296.
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Bestari, N. C., & Suharjono, S. (2015). Uji Kualitatif dan Kuantitatif Isolat Bakteri Lipolitik dari Limbah Cair Pabrik Pengolahan Ikan Kecamatan Muncar, Banyuwangi. Biotropika: Journal of Tropical Biology, 3(3), 151–155.
Cappuccino, J. G., & Sherman, N. (2014). Microbiology : A Laboratory Manual. United States of America: Pearson.
Chairunnisa, Riyanto, & Karim, A. (2020). Isolasi dan Uji Bakteri Lipolitik dalam Mendegradasi Minyak Pada Limbah Cair Kelapa Sawit di Kebun Marihat, Pematang Siantar. Jurnal Ilmiah Biologi UMA (JIBIOMA), 1(2), 44–52. https://doi.org/10.31289/jibioma.v1i2.155
Dahliaty, A., Susanti, R., & Haryani, Y. (2012). Skrining Bakteri Lipolitik dari Air Sungai Siak di Daerah Pelita Pantai Kota Pekanbaru. Indonesian Chemica Acta, 3(1), 5–8.
Dinçer, E., & Kıvanç, M. (2018). Lipolytic Activity of Lactic Acid Bacteria Isolated from Turkish Pastırma. Anadolu University Journal of Science and Technology C- Life Sciences and Biotechnology, 7(1), 12–19. https://doi.org/10.18036/aubtdc.306292
Dippenaar, M. A. (2014). Towards a Multi-Faceted Vadose Zone Assessment Protocol: Cemetery Guidelines and Application to a Burial Site Located Near a Seasonal Wetland (Pretoria, South Africa). Bulletin of Engineering Geology and the Environment, 73(4), 1105–1115. https://doi.org/10.1007/s10064-014-0635-3
Ethica, S. N. (2018). Buku Referensi Bioremediasi Limbah Biomedik Cair. Yogyakarta: Deepublish.
Evans, P. R., Whitchurch, D. M., & Muhlestein, K. (2015). Rethinking Burial Dates at a Graeco-Roman Cemetery: Fag el-Gamous, Fayoum, Egypt. Journal of Archaeological Science: Reports, 2, 209–214. https://doi.org/10.1016/j.jasrep.2015.02.004
Firdausi, W. & Zulaika, E. (2015). Potensi Azotobacter spp. sebagai Pendegradasi Lipid dan Protein. Bioeksperimen. Volume 1 No. 2. 18-21. https://doi.org/10.23917/bioeksperimen.v1i2.873.
Forbes, R. M., Cooper, A. R., & Mitchell, H. H. (1953). The Composition of the Adult Human Body as Determined by Chemical Analysis. The Journal of Biological Chemistry, 203(1), 359–366. https://doi.org/10.1016/s0021-9258(19)52646-1
Jamil, S. N. A., Wijaya, A., Sendra, E., Rahman, I. W., Chairiyah, R., Ulimaz, A., Lindawati. (2022). Mikrobiologi. Padang: Global Eksekutif Teknologi Press.
Kawuri, R., & Darmayasa, I. B. G. (2022). Potensi Bakteri Sebagai Biodegradasi Lemak dan Minyak pada Lingkungan Yang Tercemar Limbah Domestik. Metamorfosa: Journal of Biological Sciences, 9(1), 184. https://doi.org/10.24843/metamorfosa.2022.v09.i01.p18
Kaysi, A. F. El. (2018). Panduan Praktis Shalat Jenazah & Perawatan Jenazah. Yogyakarta: Media Pressindo.
Khairani, & Manalu, K. (2023). Isolasi dan Identifikasi Bakteri Lipolitik dari Limbah Cair Kelapa Sawit (Elaeis quineensis Jacq.). BIOEDUSAINS: Jurnal Pendidikan Biologi Dan Sains, 6(1), 1–13. https://doi.org/10.31539/bioedusains.v6i1.5285
Lanka, S., & Latha, J. N. L. (2015). A Short Review on Various Screening Methods to Isolate Potential Lipase Producers: Lipases-the Present and Future Enzymes of Biotech Industry. International Journal of Biological Chemistry, 9(5), 207–219. https://doi.org/10.3923/ijbc.2015.207.219
Mazhar, H., Abbas, N., Zamir, T., Hussain, Z., & Ali, S. S. (2018). Optimization Study of Lipolytic Enzyme from Bacillus cereus, PCSIR NL-37. Punjab University Journal of Zoology, 33(2), 217–224. https://doi.org/10.17582/journal.pujz/2018.33.2.217.224
Nasikhin, R., & Shovitri, M. (2013). Isolasi dan Karakterisasi Bakteri Pendegradasi Solar dan Bensin dari Perairan Pelabuhan Gresik. Jurnal Sains Dan Seni Pomits, 2(2), 84–88. http://dx.doi.org/10.12962/j23373520.v2i2.3626
Oktavia, D. A., & Wibowo, S. (2017). Penapisan dan Identifikasi Bakteri Lipolitik yang Diisolasi dari Air Limbah Pengolahan Surimi dan Pengalengan Rajungan. Jurnal Pascapanen Dan Bioteknologi Kelautan Dan Perikanan, 11(2), 147. https://doi.org/10.15578/jpbkp.v11i2.262
Pemerintah, D. Penyelenggaraan dan Pelayanan Pemakaman (2013). Magelang.
Putra, S. S., Rahayu, T., & Tyastuti, E. M. (2023). Isolation and Characterization of Cambodian Tree Rhizospheric Bacteria (Plumeria acuminata) at Pracimaloyo TPU as a producer of IAA. BIOEDUSCIENCE, 7(1), 15–23. https://doi.org/ 10.22236/jbes/7111375
Remijawa, E. S., Rupidara, A. D. N., Ngginak, J., & Radjasa, O. K. (2020). Isolasi dan Selesksi Bakteri Penghasil Enzim Ekstraseluler pada Tanah Mangrove di Pantai Noelbaki. Jurnal Enggano, 5(2), 164–180. https://doi.org/10.31186/jenggano.5.2.164-180
Rizky, M. Y., Fitri, R. D., Hastuti, U. S., & Prabaningtyas, S. (2017). Identifikasi Uji Kemampuan Hidrolisis Lemak dan Penentuan Indeks Zona Bening Asam Laktat pada Bakteri dalam Wadi Makanan Traditional Kalimantan Tengah. Bionature, 18(2), 87–98. https://doi.org/10.35580/bionature.v18i2.6137
Sayuti, I., & Suratni. (2015). Isolasi dan Identifikasi Bakteri Hidrokarbonoklastik dari Limbah Cair Minyak Bumi GS Cevron Pasifik Indonesia di Desa Benar Kecamatan Rimba Melintang Rokan Hilir. Prosiding Semirata, 4(1), 320–334.
Siregar, R. N. F., Suwandi, J. F., & Kurniati, I. (2022). Entomologi Forensik. Agromedicine, 9(1), 76–80.
Sumarlin, L. O., Mulyadi, D., Suryatna, & Asmara, Y. (2013). Identifikasi Potensi Enzim Lipase dan Selulase pada Sampah Kulit Buah Hasil Fermentasi. Jurnal Ilmu Pertanian Indonesia (JIPI), 18(3), 159–166.
Suyanto, E., Soetarto, E. S., & Cahyanto, M. N. (2015). Produk Lipase Kapang Lipolitik pada Limbah Ampas Kelapa. Bioeksperimen: Jurnal Penelitian Biologi, 1(1), 12–17. https://doi.org/10.23917/bioeksperimen.v1i1.311
Syah, M. A. (2022). Isolasi dan Karakterisasi Molekuler Gen 16S rRNA Bakteri Lipolitik Asal Limbah Kulit Biji Jambu Mete. Sumberdaya Hayati, 8(1), 20–26. https://doi.org/10.29244/jsdh.8.1.20-26
Tsani, F. F. (2021). Isolasi dan Karakteristik Bakteri Lipolitik dari Tanah Tempat Pemrosesan Akhir (TPA) Talangagung Kepanjen Kabupaten Malang. Universitas Islam Negeri Maulana Malik Ibrahim Malang.
Turajo, K. A., Abubakar, B. S. U. I., Dammo, M. N., & Sangodoyin, A. Y. (2019). Burial Practice and its Effect on Groundwater Pollution in Maiduguri, Nigeria. Environmental Science and Pollution Research, 26(23), 23372–23385. https://doi.org/10.1007/s11356-019-05572-6
Upayogi, I. N. T. (2019). Kajian Eksplanasi Taru Menyan Penetral Bau Mayat. Jurnal Filsafat Indonesia, 2(1), 37. https://doi.org/10.23887/jfi.v2i1.17550
Widiastuti, L., Sulistiyanto, Y., Jaya, A., Jagau, Y., & Neneng, L. (2019). Potensi Mikroorganisme Sebagai Biofertilizer Dari Limbah Cair Pabrik Kelapa Sawit. Jurnal Surya Medika, 5(1), 1–12. https://doi.org/10.33084/jsm.v5i1.941
Yousef, A. E., Cusic, J. G. W., & Perry, J. J. (2022). Analytical Food Microbiology: A Laboratory Manual. Amerika Serikat: Wiley.
Zainuddin, Habib, Z., Rachmadhani, A., Nuriyanto, K., Wibowo, A. M., & Solikin, N. (2022). The Impact Of State Regulations On Social Changes Futures As Cultural Spaces. Journal of Positive School Psychology, 6(6), 4281–4296.
Authors
Mandiri, L. H., Rahayu, T., Tyastuti, E. M., & Sidiq, Y. (2023). Screening of Lipolytic Bacteria from Cemetery Soil. BIOEDUSCIENCE, 7(3), 358–364. https://doi.org/10.22236/jbes/12686
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