Test of Hypersensitivity and Antagonistic Reaction of Endophytic Bacteria from Klutuk Banana (Musa balbisiana)

Ratih Dewianty, Triastuti Rahayu, Yasir Sidiq

Abstract

Background: Plants’ response and antagonistic reaction against disease-causing organisms are two crucial characteristics of Plant Growth Promoting Bacteria (PGPB). Seventeen endophytic bacteria have been isolated from Musa balbisiana. However, the plants’ reaction against these isolates and their antagonistic activities against disease-causing organisms remained unknown. This study aims to (1) determine the sensitivity effect of endophytic bacteria isolates to tobacco and (2) test the antagonism of endophytic bacteria isolates against the blast disease actor, namely Pyricularia oryzae. Method: Sensitivity tests were performed by inoculating bacterial isolates into tobacco leaves with infiltration. Bacterial isolates were prepared with a minimum OD 600: 0.5. Then, a milliliter volume of each isolate was infiltrated into tobacco leaves from the abaxial side using a 3 mL syringe. The lesion on the tobacco leaves was observed seven days after inoculation (DAI). The antagonism test was carried out by growing Pyricularia oryzae on a PDA plate for seven days, subsequently transferred to an NA medium for three days then inoculated with endophytic bacteria. The assessed parameter of the antagonistic test was the formation of an inhibitory zone between endophytic bacteria and Pyricularia oryzae at 4 DAI. Results: No hypersensitive reaction of tobacco leaves against K117, K324, K38, K86, K18, K28, K102 isolates inoculation at 7 DAI. Furthermore, the antagonistic test indicated that all isolates inhibited the growth of Pyricularia oryzae, with the range of inhibition from 32.36 to 40.46%. Implication: Thus, these results revealed the PGPB characteristics in the newly isolated endophytic bacteria from the banana.

Full text article

Generated from XML file

References

Anasari, S., Nurdin, M., Ivayani, & Ratih, S. (2022). Eksplorasi Mikroorganisme Prokariot Asal Bonggol Pisang Untuk Mengendalikan Penyakit Layu Bakteri (Ralstonia solanacearum smith) Tanaman Pisang Secara In Vitro. Jurnal Agrotek Tropika, 10(3), 461–468.
Asmoro, P. P., & Munif, A. (2020). Bakteri Endofit Dari Tumbuhan Paku-Pakuan Sebagai Agens Hayati Rhizoctonia Solani Dan Pemacu Pertumbuhan Tanaman Padi. Jurnal Fitopatologi Indonesia, 15(6), 239–247. Https://doi.org/10.14692/Jfi.15.6.239-247
Dewa Gede Wiryangga Selangga & Listihani. (2021). Screening Of Endophytic Bacteria Isolated From Mimosa Pudica In Bali Island. SEAS (Sustainable Environment Agricultural Science), 5(1), 50–57. Https://doi.org/10.22225/Seas.5.1.3303.50-57
Eka Kusumawati, D., & Istiqomah, I. (2020). Potensi Agensia Hayati Dalam Menekan Laju Serangan Penyakit Blas (Pyricularia oryzae) Pada Tanaman Padi. VIABEL: Jurnal Ilmiah Ilmu-Ilmu Pertanian, 14(2), 1–13. Https://doi.org/10.35457/Viabel.V14i2.1235
Eris, D. D., Munif, A., Soekarno, B. P., & Purwantara, A. (2018). Antagonisme Beberapa Bakteri Endofit Arecaceae Terhadap Curvularia Sp. Patogen Penyebab Bercak Daun Yang Diisolasi Dari Tanaman Kelapa Kopyor.
Fajarfika, R., Hilmany, T., Nafi’ah, H. H., Sativa, N., & Supriatna, J. (2022). Isolasi Pseudomonas sp. Untuk Pengendalian Biologi Terhadap Layu Bakteri. 6(2).
Flori, F. (2020). Potensi Antagonis Isolat Bakteri Bacillus Spp. Asal Rizosfer Tanaman Lada (Piper nigrum L.) Sebagai Agen Pengendali Jamur Fusarium sp. JDF. 5.
Hastuti, D. D., Saylendra, A., & Rohman, E. S. (2014). Skrining Bakteri Endofit Perakaran Pisang Secara In Vitro Sebagai Agen Pengendali Hayati Terhadap Penyakit Layu Bakteri (Ralstonia Solanacearum) Pada Tanaman Pisang. Jurnal Agroekoteknologi, 6(1).
Indaryaningsih, N., Sektiono, A. W., & Sastrahidayat, I. R. (2021). Identifikasi Penyakit Hawar Daun Pada Drasena (Dracaena sp.). Serta Uji Penghambatannya Menggunakan Jamur Antagonis Secara In Vitro. Jurnal Hama Dan Penyakit Tumbuhan, 9(2), 65–71. Https://doi.org/10.21776/Ub.Jurnalhpt.2021.009.2.5
Lamsal, K., Kim, S. W., Kim, Y. S., & Lee, Y. S. (2012). Application of Rhizobacteria For Plant Growth Promotion Effect And Biocontrol Of Anthracnose Caused By Colletotrichum Acutatum On Pepper. Mycobiology, 40(4), 244–251. Https://doi.org/10.5941/MYCO.2012.40.4.244
Lestari, S. A., Ramdan, E. P., Universitas Gunadarma, Kulsum, U., & Balai Besar Peramalan Organisme Pengganggu Tumbuhan. (2021). Identifikasi Penyebab Penyakit Blas Padi Pada Kombinasi Pola Tanam System of Rice Intensification (SRI) Dan Jajar Legowo. Peningkatan Produktivitas Pertanian Era Society 5.0 Pasca Pandemi, 312–321. Https://doi.org/10.25047/Agropross.2021.235
Marsaoli, F., Matinahoru, J. M., & Leiwakabessy, Ch. (2020). Isolasi, Seleksi, Dan Uji Antagonis Bakteri Endofit Diisolasi Dari Salawaku (Falcataria mollucana) dalam Menekan Pertumbuhan Cendawan Patogen Cercospora sp. Agrologia, 8(2). Https://doi.org/10.30598/A.V8i2.1009
Marwan, H., Nusifera, S., & Mulyati, S. (2021). Potensi Bakteri Endofit Sebagai Agens Hayati Untuk Mengendalikan Penyakit Blas Pada Tanaman Padi. Jurnal Ilmu Pertanian Indonesia, 26(3), 328–333. Https://doi.org/10.18343/Jipi.26.3.328
Nawangsih, A. A. (2007). Pemanfaatan Bakteri Endofit Pada Pisang Untuk Mengendalikan Penyakit Darah: Isolasi, Uji Penghambatan In Vitro Dan In Planta. Jurnal Ilmu Pertanian Indonesia, 12(1), 43-49.
Nakkeeran, S., Rajamanickam, S., Saravanan, R., Vanthana, M., & Soorianathasundaram, K. (2021). Bacterial Endophytome Mediated Resistance in Banana for The Management of Fusarium Wilt. Biotech, 11(267), 1–13.
Oktafiyanto, M. F., Munif, A., & Mutaqin, K. H. (2018). Aktivitas Antagonis Bakteri Endofit Asal Mangrove Terhadap Ralstonia Solanacearum Dan Meloidogyne Spp. Jurnal Fitopatologi Indonesia, 14(1), 23. Https://doi.org/10.14692/Jfi.14.1.23
Parida, I., Damayanti, T. A., & Giyanto, G. (2017). Isolasi, Seleksi, Dan Identifikasi Bakteri Endofit Sebagai Agens Penginduksi Ketahanan Padi Terhadap Hawar Daun Bakteri. Jurnal Fitopatologi Indonesia, 12(6), 199. Https://doi.org/10.14692/Jfi.12.6.199
Pitasari, A., & Ali, M. (2018). Tanaman Bawang Merah (Allium ascalonicum L.) Terhadap Jamur Alternaria Porri Ellis Cif. 5(1).
Pulungan, A. S. S., & Tumangger, D. E. (2018). Isolasi Dan Karakterisasi Bakteri Endofit Penghasil Enzim Katalase Dari Daun Buasbuas (Premna pubescens blume). BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan), 5(1), 71. https://doi.org/10.31289/Biolink.V5i1.1665
Rahayu, T., Purwestri, Y. A., Subandiyah, S., & Widianto, D. (2021). Potensi Bakteri Endofit Asal Tanaman Pisang Klutuk (Musa balbisiana Colla) Sebagai Pendukung Pertumbuhan Tanaman. Al-Kauniyah: Jurnal Biologi, 14(2), 313–324. Https://doi.org/10.15408/Kauniyah.V14i2.19140
Ramdan, E. P., Maghfiroh, E. L., Rinika, R., & Munif, A. (2021). Selection of Endophytic Bacteria From Root Of Okra (Abelmoschus esculantus) As A Biocontrol Of Fusarium Oxysporum. Gontor AGROTECH Science Journal, 7(1), 1. https://doi.org/10.21111/Agrotech.V7i1.4545
Ramses, Fenny Agustina, R. Pramuanggit Panggih Nugroho. (2021) Antibacterial Potential of Bidara Laut (Ximenia americana) Plant Against Vibrio alginolyticus and V. parahaemolyticus Bacteria. BIOEDUSCIENCE, 5(1), 15-23. https://doi.org/10.22236/j.bes/515091
Ruslan, R., Ismed, F., & Nabila, G. S. (2022). Uji Aktivitas Antibakteri Dari Ekstrak Bakteri Endofit yang Diisolasi Dari Kulit Jeruk Nipis. Jurnal Sains Farmasi & Klinis, 9(1), 42. Https://doi.org/10.25077/Jsfk.9.1.42-49.2022
Sadikin, N. A. N., Bintari, S. H., Widiatningrum, T., & Dewi, P. (2021). Isolasi, Karakterisasi, Dan Uji Aktivitas Antibakteri Dari Bakteri Endofit Daun Kelor (Moringa Oleifera). Life Science, 10(2), 109–119. Https://doi.org/10.15294/Lifesci.V10i2.54441
Savani, A. Kumar, Bhattacharyya, A., Boro, Obin C., Dinesh, K, & Jc, N. S. (2021). Exemplifying Endophytes of Banana (Musa paradisiaca) For Their Potential Role in Growth Stimulation and Management Of Fusarium Oxysporum F. Sp Cubense Causing Panama Wilt. Folia Microbiologica, 5(5), 1–14.
Sucipto, I., Munif, A., Institut Pertanian Bogor, Indonesia, Suryadi, Y., Balai Besar Penelitian Dan Pengembangan Bioteknologi Dan Sumber Daya Genetik Pertanian, Bogor, Indonesia, Tondok, E. T., & Institut Pertanian Bogor, Indonesia. (2015). Eksplorasi Cendawan Endofit Asal Padi Sawah Sebagai Agens Pengendali Penyakit Blas Pada Padi Sawah. Jurnal Fitopatologi Indonesia, 11(6), 211–218. https://doi.org/10.14692/Jfi.11.6.211
Sunariasih, N. P. L., Suada, I. K., & Suniti, N. W. (2014a). Identifikasi Jamur Endofit Dari Biji Padi Dan Uji Daya Hambatnya Terhadap Pyricularia Oryzae Cav. Secara In Vitro. 3(2).
Sunariasih, N. P. L., Suada, I. K., & Suniti, N. W. (2014b). Identifikasi Jamur Endofit Dari Biji Padi Dan Uji Daya Hambatnya Terhadap Pyricularia Oryzae Cav. Secara In Vitro. 3(2).
Suryadi, Y., Priyatno, T., Susilowati, D., Samudra, I., Yudhistira, N., & Purwakusumah, E. (N.D.). Isolasi Dan Karakterisasi Kitinase Asal Bacillus Cereus 11 UJ (Isolation and Chitinase Characterization Of Bacillus Cereus 11 UJ).
Wibowo, R. H., Sipriyadi, S., Mubarik, N. R., & Rusmana, I. (2020). Soil Chitinolytic Bacteria from Jambi Province to Produce Antifungal Of Plant Pathogens. Mangifera Edu, 5(1), 26–37. Https://doi.org/10.31943/Mangiferaedu.V5i1.95
Wiratno, W., Syakir, M., Sucipto, I., & Pradana, A. P. (2019). Isolation and Characterization Of Endophytic Bacteria From Roots Of Piper Nigrum And Their Activities Against Fusarium Oxysporum And Meloidogyne Incognita. Biodiversitas Journal Of Biological Diversity, 20(3), 682–687. Https://doi.org/10.13057/Biodiv/D200310
Wulandari, H. (2012). Isolation and Antagonistic Test of Endophytic Bacteria from Pepper. 2(2), 9.
Yatni, Y., Tuhumury, G. N. C., & Leiwakabessy, C. (2018). Potensi Bakteri Endofit Dari Tanaman Sagu (Metroxylon Spp.) Sebagai Agens Pemacu Pertumbuhan Tanaman Padi. JURNAL BUDIDAYA PERTANIAN, 14(2), 75–80. Https://doi.org/10.30598/Jbdp.2018.14.2.75
Zulaika, Z., Soekarno, B. P., & Nurmansyah, A. (2018). Pemodelan Keparahan Penyakit Blas Pada Tanaman Padi Di Kabupaten Subang. Jurnal Fitopatologi Indonesia, 14(2), 47. Https://doi.org/10.14692/Jfi.14.2.47
Zuraidah, Z., Nida, Q., & Wahyuni, S. (2020). Uji Antagonis Bakteri Terhadap Cendawan Patogen Penyakit BlaS. BIOTIK: Jurnal Ilmiah Biologi Teknologi Dan Kependidikan, 8(1), 37. Https://doi.org/10.22373/Biotik.V8i1.6667

Authors

Ratih Dewianty
Triastuti Rahayu
Yasir Sidiq
ys120@ums.ac.id (Primary Contact)
Dewianty, R., Rahayu, T., & Sidiq, Y. (2023). Test of Hypersensitivity and Antagonistic Reaction of Endophytic Bacteria from Klutuk Banana (Musa balbisiana). BIOEDUSCIENCE, 7(2), 222–230. https://doi.org/10.22236/jbes/11795

Article Details