Screening and Identification of Cellulolytic Bacteria from Public Cemetery Soil
Abstract
Background: A public cemetery (TPU) is where bodies are buried. Before being buried, the body is wrapped in a shroud or full clothes and sometimes put in a coffin. These materials contain cellulose, a substrate for cellulolytic microorganisms, including bacteria, to decompose. Exploration of cellulolytic bacteria in TPU has not been found, even though TPU holds potential cellulolytic bodies. This study aims to determine the cellulolytic potential and identify bacterial isolates from TPU. Methods: 36 bacterial isolates tested for cellulolytic potential have been isolated from TPU Pracimaloyo Kartasura, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripped with Congo red 0.1%, while identification was based on colony morphology and Gram staining. Results: This study obtained one isolate (2.78%) showing high cellulolytic activity with a cellulolytic index (IS) 7, namely P14, 24 isolates (66.67%) in the "moderate" category, four isolates (11.11%) with category "low," and seven isolates (19.44%) did not show cellulolytic ability. Isolates cellulolytic positive have a shiny white colony color, entire edges, raised elevation, and belong to the Gram-negative coccus form. Conclusions: From the results of this study, it can be concluded that public burial sites (TPU) harbor potential cellulolytic bacteria.
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Diputra, M., & Syaodih, E. (2017). Prosiding Perencanaan Wilayah dan Kota Identifikasi Permasalahan Tempat Pemakaman Umum di Kota Bandar Lampung. Prosiding Perencanaan Wilayah Dan Kota, 3(2), 283–291. http://dx.doi.org/10.29313/pwk.v0i0.6698
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Kartika, I. N., & Ibrahim, M. (2021). Efek Manipulasi pH pada Aktivitas Enzim Selulase Bakteri Bacillus subtillis Strain FNCC 0059 dalam Mendegradasi Selulosa (Effects of pH Manipulation on the Activity of the Cellulase Enzyme Bacteria Bacillus subtillis Strain FNCC 0059 in Degrade Cellulose). LenteraBio, 10(1), 51–57. https://doi.org/10.26740/lenterabio.v10n1.p51-57
Khotimah, S. Suharjono. Asdyati, T. & Nurani, Y. (2020). Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia. Biodiversitas. 21(5), 2103-2122. https://DOI: 10.13057/biodiv/d210538
Lengkong, S. C., Siahaan, P., & Tangapo, A. M. (2022). Analisis Karakteristik dan Uji Bioaktivitas Bakteri Rizosfer PGPR (Plant Growth Promoting Rhizobacteria) Isolat Kalasey. JURNAL BIOS LOGOS, 12(2), 104. https://doi.org/10.35799/jbl.v12i2.42131
Mifbakhuddin, Ayu, L, Nurullita, U. (2022). Pengaruh Frekuensi Penyiraman Air Limbah Cucian Beras terhadap Lama Waktu Pengomposan dengan Metode Biopori. Jurnal Kesehatan. 15(1), 45-50. DOI: 10.23917/jk.v15i1.16517.
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Rahayu, T. Asngad, A. Suparti. (2017). Biopulping Pelapah Tanaman Salak menggunakan Jamur Pelapuk Putih Phanerochaete chrysosporium. Bioeksperimen: Jurnal Pendidikan Biologi. Volume 3 No.1. https://doi.org/10.23917/bioeksperimen.v3i1.3671.
Sharma S, Kumawat KC, Kaur S. (2022). Potential of indigenous ligno-cellulolytic microbial consortium to accelerate degradation of heterogenous crop residues. Environ Sci Pollut Res Int. 29(58):88331-88346. doi: 10.1007/s11356-022-21809-3. Epub 2022 Jul 14. PMID: 35834084.
Satwika, T. D., Yulianti, D. M., & Hikam, A. R. (2021). Karakteristik dan Potensi Enzimatis Bakteri Asal Tanah Sampah Dapur dan Kotoran Ternak sebagai Kandidat Agen Biodegradasi Sampah Organik. Al-Hayat: Journal of Biology and Applied Biology, 4(1), 11–18. https://doi.org/10.21580/ah.v4i1.7013
Shajahan, S., Moorthy, I. G., Sivakumar, N., & Selvakumar, G. (2017). Statistical modeling and optimization of cellulase production by Bacillus licheniformis NCIM 5556 isolated from the hot spring, Maharashtra, India. Journal of King Saud University - Science, 29(3), 302–310. https://doi.org/10.1016/j.jksus.2016.08.001
Tearher, R. M., & Wood, P. J. (1982). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Applied and Environmental Microbiology, 43(4), 777–780. https://doi.org/10.1128/aem.43.4.777-780.1982
Yusnia, E. D., Bagus, I., Gunam, W., & Antara, N. S. (2019). Isolasi dan Skrining Bakteri Selulolitik dari Beberapa Tanah Hutan di Bali (Isolation and screening cellulolytic bacteria from some soil forest in Bali).(Vol. 7, Issue 1). https://www.academia.edu/download/65816174/28596.pdf
Zubaidah, A., Prasetyo, D., Handajani, H., Rohmah, S. P., & Puspita, D. A. (2019). Screening Bakteri Selulolitik dan Amilolitik Pada Rumen Sapi Sebagai Kandidat Probiotik Pada Budidaya Ikan Secara In Vitro. Jurnal Riset Akuakultur, 14(4), 261–2711. http://ejournal-balitbang.kkp.go.id/index.php/jra/article/view/7998/6509
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Agustriono, F. R., & Hasaanah, A. N. (2016). Pemanfaatan Limbah Sebagai Bahan Baku Sintesis Karboksimetil Selulosa : Review. Farmaka, 14(3), 87–94. https://doi.org/10.24198/jf.v14i3.10788
Akmala, A., & Supriyo, E. (2022). Optimasi Konsentrasi Selulosa pada Pembuatan Biodegradable Foam dari Selulosa dan Tepung Singkong. Pentana: Jurnal Penelitian Terapan Kimia, 1(1), 27–40. https://ejournal2.undip.ac.id/index.php/pentana/article/view/11597
Andriani, A. A. S. P. R., Dewi, W. S., Nazir, N., Setianingsih, N. L. P. P., Indrayatie, E. R., & Kalimutu, P. K. (2023). Characteristics of Indigenous Bacterial Isolates from Cocoa Plantations in Meko Village, Central Sulawesi, with Ability to Degrade Cellulose. Asian Journal of Applied Research for Community Development and Empowerment, 7(2). https://doi.org/10.29165/ajarcde.v7i2.256
Apun, K., Jong, B. C., & Salleh, M. A. (2000). Screening and isolation of a cellulolytic and amylolytic Bacillus from sago pith waste. J. Gen. Appl. Microbiol, 46, 263–267. https://www.jstage.jst.go.jp/article/jgam/46/5/46_5_263/_pdf/-char/en
Dar, M. A., Pawar, K. D., Jadhav, J. P., & Pandit, R. S. (2015). Isolation of cellulolytic bacteria from the gastro-intestinal tract of Achatina fulica (Gastropoda: Pulmonata) and their evaluation for cellulose biodegradation. International Biodeterioration and Biodegradation, 98, 73–80. https://doi.org/10.1016/j.ibiod.2014.11.016
Diputra, M., & Syaodih, E. (2017). Prosiding Perencanaan Wilayah dan Kota Identifikasi Permasalahan Tempat Pemakaman Umum di Kota Bandar Lampung. Prosiding Perencanaan Wilayah Dan Kota, 3(2), 283–291. http://dx.doi.org/10.29313/pwk.v0i0.6698
Ejaz, U., Sohail, M., & Ghanemi, A. (2021). Cellulases: From bioactivity to a variety of industrial applications. In Biomimetics (Vol. 6, Issue 3). MDPI AG. https://doi.org/10.3390/biomimetics6030044
Fauziah, S. I., & Ibrahim, M. (2020). Isolasi dan Karakterisasi Bakteri Selulolitik pada Tanah Gambut di Desa Tagagiri. LenteraBio, 9(3), 194–203. https://doi.org/10.26740/lenterabio.v9n3.p194-203
Hartanti, D. A. S. (2020). Isolasi dan uji sinergisme bakteri endofit tanaman padi (Oryza sativa L.) Untuk konsorsium biofertilizer. Agroradix, 3(2), 23–30. https://doi.org/10.52166/agroteknologi.v3i2.1951
Hidayat, H. (2011). Karakterisasi Molekuler BAL Dengan Gen 16S rRNA Penghasil ENzim Protease Yang berpotensi sebagai Probiotik Dari Fermentasi Markisa Kuning di Sumatera Barat [Tesis]. Universitas Andalas .
Irianto, K. (2014). Bakteriologi, Mikologi, Virologi (K. Irianto, Ed.). Alfabeta.
Karthika, A. Seenivasagan, R. Kasimani, R. Babalola, O.O. Vasanthy, M. (2020). Cellulolytic bacteria isolation, screening and optimization of enzyme production from vermicompost of paper cup waste, Waste Management, Volume 116, Pages 58-65, https://doi.org/10.1016/j.wasman.2020.06.036.
Kartika, I. N., & Ibrahim, M. (2021). Efek Manipulasi pH pada Aktivitas Enzim Selulase Bakteri Bacillus subtillis Strain FNCC 0059 dalam Mendegradasi Selulosa (Effects of pH Manipulation on the Activity of the Cellulase Enzyme Bacteria Bacillus subtillis Strain FNCC 0059 in Degrade Cellulose). LenteraBio, 10(1), 51–57. https://doi.org/10.26740/lenterabio.v10n1.p51-57
Khotimah, S. Suharjono. Asdyati, T. & Nurani, Y. (2020). Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia. Biodiversitas. 21(5), 2103-2122. https://DOI: 10.13057/biodiv/d210538
Lengkong, S. C., Siahaan, P., & Tangapo, A. M. (2022). Analisis Karakteristik dan Uji Bioaktivitas Bakteri Rizosfer PGPR (Plant Growth Promoting Rhizobacteria) Isolat Kalasey. JURNAL BIOS LOGOS, 12(2), 104. https://doi.org/10.35799/jbl.v12i2.42131
Mifbakhuddin, Ayu, L, Nurullita, U. (2022). Pengaruh Frekuensi Penyiraman Air Limbah Cucian Beras terhadap Lama Waktu Pengomposan dengan Metode Biopori. Jurnal Kesehatan. 15(1), 45-50. DOI: 10.23917/jk.v15i1.16517.
Murtiyaningsih, H., & Hazmi, M. (2017). Isolasi Dan Uji Aktivitas Enzim Selulase Pada Bakteri Selulolitik Asal Tanah Sampah Isolation And Cellulase Enzyme Activities Assays In Cellulolytic Bacteria Origin From Soil Waste. In 135 Agritrop (Vol. 15, Issue 2). http://jurnal.unmuhjember.ac.id/
Puspitasari, D., & Ibrahim, M. (2020). Optimasi Aktivitas Selulase Ekstraseluler Islolat Bakter EG 2 Isolasi dari Bungkil Kelapa Sawit (Elaesis guineensis jacq.) Optimization of EExtracelluler Cellulase Activity of Isolate Bacterial EG 2 Isolated from Palm Oilcake (Elaesis guineensis jacq.). LenteraBio, 9(1), 42–50. https://journal.unesa.ac.id/index.php/lenterabio/index42
Putra, S. S., Rahayu, T., & Tyastuti, E. M. (2023). Isolation and Characterization of Cambodian Tree Rhizosphere Bacteria (Plumeria acuminata) at TPU Pracimaloyo as a producer of IAA. Bioeduscience, 7(1), 15–23. https://doi.org/10.22236/jbes/7111375.
Rahayu, T. Asngad, A. Suparti. (2017). Biopulping Pelapah Tanaman Salak menggunakan Jamur Pelapuk Putih Phanerochaete chrysosporium. Bioeksperimen: Jurnal Pendidikan Biologi. Volume 3 No.1. https://doi.org/10.23917/bioeksperimen.v3i1.3671.
Sharma S, Kumawat KC, Kaur S. (2022). Potential of indigenous ligno-cellulolytic microbial consortium to accelerate degradation of heterogenous crop residues. Environ Sci Pollut Res Int. 29(58):88331-88346. doi: 10.1007/s11356-022-21809-3. Epub 2022 Jul 14. PMID: 35834084.
Satwika, T. D., Yulianti, D. M., & Hikam, A. R. (2021). Karakteristik dan Potensi Enzimatis Bakteri Asal Tanah Sampah Dapur dan Kotoran Ternak sebagai Kandidat Agen Biodegradasi Sampah Organik. Al-Hayat: Journal of Biology and Applied Biology, 4(1), 11–18. https://doi.org/10.21580/ah.v4i1.7013
Shajahan, S., Moorthy, I. G., Sivakumar, N., & Selvakumar, G. (2017). Statistical modeling and optimization of cellulase production by Bacillus licheniformis NCIM 5556 isolated from the hot spring, Maharashtra, India. Journal of King Saud University - Science, 29(3), 302–310. https://doi.org/10.1016/j.jksus.2016.08.001
Tearher, R. M., & Wood, P. J. (1982). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Applied and Environmental Microbiology, 43(4), 777–780. https://doi.org/10.1128/aem.43.4.777-780.1982
Yusnia, E. D., Bagus, I., Gunam, W., & Antara, N. S. (2019). Isolasi dan Skrining Bakteri Selulolitik dari Beberapa Tanah Hutan di Bali (Isolation and screening cellulolytic bacteria from some soil forest in Bali).(Vol. 7, Issue 1). https://www.academia.edu/download/65816174/28596.pdf
Zubaidah, A., Prasetyo, D., Handajani, H., Rohmah, S. P., & Puspita, D. A. (2019). Screening Bakteri Selulolitik dan Amilolitik Pada Rumen Sapi Sebagai Kandidat Probiotik Pada Budidaya Ikan Secara In Vitro. Jurnal Riset Akuakultur, 14(4), 261–2711. http://ejournal-balitbang.kkp.go.id/index.php/jra/article/view/7998/6509
Authors
Syarifah, S. N., Rahayu, T., Tyastuti, E. M., & Sidiq, Y. (2023). Screening and Identification of Cellulolytic Bacteria from Public Cemetery Soil. BIOEDUSCIENCE, 7(2), 192–199. https://doi.org/10.22236/jbes/11745
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