Isolation and Characterization of Cambodian Tree Rhizospheric Bacteria (Plumeria acuminata) at Pracimaloyo TPU as a producer of IAA
Abstract
Background: Cambodian trees are known to be resistant to biotic and abiotic stresses which may be influenced by the presence of rhizosphere bacteria as Plant Growth-Promoting Rhizobacteria (PGPR). This study aims to isolate and characterize Cambodian tree rhizosphere bacteria from burial soils that have the potential to produce IAA. Methods: Rhizospheric soil samples were taken from Pracimaloyo TPU, Surakarta, Central Java, at 5 points attached to the surface of the frangipani tree roots to be inoculated using the scattering cup method at 10-5 and 10-6 dilutions in NA (nutrient agar). After 48 hours, colonies were counted to obtain population data. The ability of rhizosphere bacteria to produce IAA was carried out qualitatively and quantitatively at the age of 24 and 48 hours of culture. Rhizospheric bacteria isolates potential to produce IAA were characterized macroscopically (colony morphology) and microscopically by Gram staining. Results: The population of frangipani tree rhizosphere bacteria in all blocks did not show a significant difference and was detected to have a population between 1.9 – 10.4 x 106. Qualitative test of the ability to produce IAA, it was detected that 34.88% of isolates produced very high IAA. The highest concentration of IAA was produced by isolate P37, followed by P38 and P24 at 48 hours of age, namely 113.58 ppm, 77.95 ppm, and 55.69 ppm. All potential isolates to produce IAA are cocci-shaped gram-negative bacteria. Conclusion: The population of frangipani tree rhizosphere bacteria in Pracimaloyo TPU ranged from 1.9-10.4 x 106 CFU/g and 34.88% had the ability to produce IAA. The concentration of IAA produced was higher at 48 hours of culture compared to 24 hours with the highest concentration by isolate P37 (83.098 ppm and increased to 113.588 ppm). Isolate P37 is a gram-negative cocci-shaped bacterium and irregular colonies.
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References
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Devi, A. R., Susilowati, A., & RATNA SETYANINGSIH. (2019). Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas. Biodiversitas Journal of Biological Diversity, 20(10). https://doi.org/10.13057/biodiv/d201016
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Hengkengbala, S. I., Lintang, R. A., Sumilat, D. A., Mangindaan, R. E., Ginting, E. L., & Tumembouw, S. (2021). Karakteristik Morfologi Dan Aktivitas Enzim Protease Bakteri Simbion Nudibranch. Jurnal Pesisir Dan Laut Tropis, 9(3), 83. https://doi.org/10.35800/jplt.9.3.2021.36672
Hutapea, V. H. (2023). Isolasi dan Karakterisasi Bakteri Penghasil Iaa (Indole Acetic Acid) dari Rhizosfer Tanaman Karet (Hevea brasiliensis Mull. Arg.) [Skripsi]. Universitas Sebelas Maret.
Jacoby, R., Peukert, M., Succurro, A., Koprivova, A., & Kopriva, S. (2017). The Role of Soil Microorganisms in Plant Mineral Nutrition—Current Knowledge and Future Directions. Frontiers in Plant Science, 8. https://doi.org/10.3389/fpls.2017.01617
Jeyanthi, V., & Kanimozhi, S. (2018). Plant growth promoting rhizobacteria (PGPR)-prospective and mechanisms: a review. J Pure Appl Microbiol, 12(2), 733–749.
Kandel, S., Joubert, P., & Doty, S. (2017). Bacterial Endophyte Colonization and Distribution within Plants. Microorganisms, 5(4), 77. https://doi.org/10.3390/microorganisms5040077
Kochar, M., Upadhyay, A., arun, vaishnavi, & Srivastava, S. (2013). Bacterial Biosynthesis of Indole-3-Acetic Acid: Signal Messenger Service. In Molecular Microbial Ecology of the Rhizosphere (Vol. 1). https://doi.org/10.1002/9781118297674.ch29
Laskow, S. (2018). There’s a bunch of gross stuff, besides human bodies, hiding under graveyards, URL: https://www.atlasobscura.com/articles/cemetery-soil-human-remains (accessed on 23 March 2023).
Lewis, K. (2000). Programmed Death in Bacteria. Microbiology and Molecular Biology Reviews, 64(3), 503–514. https://doi.org/10.1128/MMBR.64.3.503-514.2000
Lubis, A. F. (2021). Keanekaragaman Organisme Tanah pada Kelapa Sawit di Daerah Endemik Ganoderma boninense Pat. di Kebun Tanjung Beringin, Langkat [Tesis]. Universitas Sumatera Utara.
Lumombo, D. T. (2017). Kajian Yuridis Peraturan Pemerintah Republik Indonesia Nomor 9 Tahun 1987 Tentang Penyediaan Dan Penggunaan Tanah Untuk Keperluan Tempat Pemakaman. Lex Crimen, 6(8).
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Nuraini, A. N., Aisyah, & Ramdan, E. P. (2020). Seleksi Bakteri Rhizosfer Tanaman Rambutan Sebagai Agens Biokontrol Penyakit Antraknosa Pada Cabai (Capsicum annum L.). Jurnal Pertanian Presisi (Journal of Precision Agriculture), 4(2), 100–112. https://doi.org/10.35760/jpp.2020.v4i2.2999
Orozco-Mosqueda, Ma. del C., Glick, B. R., & Santoyo, G. (2020). ACC deaminase in plant growth-promoting bacteria (PGPB): An efficient mechanism to counter salt stress in crops. Microbiological Research, 235, 126439. https://doi.org/10.1016/j.micres.2020.126439
Rahayu, T., Purwestri, Y. A., Subandiyah, S., & Widianto, D. (2021). Potensi Bakteri Endofit Asal Tanaman Pisang Klutuk (Musa balbisiana Colla) Sebagai Pendukung Pertumbuhan Tanaman. Al-Kauniyah: Jurnal Biologi, 14(2), 313–324.
Ristiari, N. P. N., Julyasih, K. S. M., & Suryanti, I. A. P. (2019). Isolasi dan identifikasi jamur mikroskopis pada rizosfer tanaman jeruk siam (Citrus nobilis Lour.) di Kecamatan Kintamani, Bali. Jurnal Pendidikan Biologi Undiksha, 6(1), 10–19.
Santoyo, G., Moreno-Hagelsieb, G., del Carmen Orozco-Mosqueda, Ma., & Glick, B. R. (2016). Plant growth-promoting bacterial endophytes. Microbiological Research, 183, 92–99. https://doi.org/10.1016/j.micres.2015.11.008
Sudewi, S., Ala, A., Patandjengi, B., Bdr, M. F., & Rahim, A. (2020). Scereening of Plant Growth Promotion Rhizobacteria (PGPR) to increase local aromatic rice plant growth. Int. J. Pharm. Res, 13.
Suliasih, & Widawati, S. (2020). Isolation of Indole Acetic Acid (IAA) producing Bacillus siamensis from peat and optimization of the culture conditions for maximum IAA production. IOP Conference Series: Earth and Environmental Science, 572(1), 012025.
Vejan, P., Abdullah, R., Khadiran, T., Ismail, S., & Nasrulhaq Boyce, A. (2016). Role of Plant Growth Promoting Rhizobacteria in Agricultural Sustainability—A Review. Molecules, 21(5), 573. https://doi.org/10.3390/molecules21050573
Walida, H., Harahap, F. S., Hasibuan, M., & Yanti, F. F. (2019). Isolasi dan identifikasi bakteri penghasil IAA dan pelarut fosfat dari rhizosfer tanaman kelapa sawit. BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan), 6(1), 1–7.
Wallenstein, M. (2017, July 28). To restore our soils, feed the microbes, URL: https://theconversation.com/to-restore-our-soils-feed-the-microbes-79616 (accessed on 23 March 2023).
Xu, L., & Liu, Y. (2014). Protein secretion systems in bacterial pathogens. Frontiers in Biology, 9(6), 437–447. https://doi.org/10.1007/s11515-014-1333-z
Zarei, T., Moradi, A., Kazemeini, S. A., Akhgar, A., & Rahi, A. A. (2020). The role of ACC deaminase producing bacteria in improving sweet corn (Zea mays L. var saccharata) productivity under limited availability of irrigation water. Scientific Reports, 10(1), 20361. https://doi.org/10.1038/s41598-020-77305-6
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Backer, R., Rokem, J. S., Ilangumaran, G., Lamont, J., Praslickova, D., Ricci, E., Subramanian, S., & Smith, D. L. (2018). Plant Growth-Promoting Rhizobacteria: Context, Mechanisms of Action, and Roadmap to Commercialization of Biostimulants for Sustainable Agriculture. Frontiers in Plant Science, 9. https://doi.org/10.3389/fpls.2018.01473
Bais, H. P., Weir, T. L., Perry, L. G., Gilroy, S., & Vivanco, J. M. (2006). The Role Of Root Exudates In Rhizosphere Interactions With Plants And Other Organisms. Annual Review of Plant Biology, 57(1), 233–266. https://doi.org/10.1146/annurev.arplant.57.032905.105159
Cruz, D., Cisneros, R., Benítez, Á., Zúñiga-Sarango, W., Peña, J., Fernández, H., & Jaramillo, A. (2021). Gram-Negative Bacteria from Organic and Conventional Agriculture in the Hydrographic Basin of Loja: Quality or Pathogen Reservoir? Agronomy, 11(11), 2362. https://doi.org/10.3390/agronomy11112362
Devi, A. R., Susilowati, A., & RATNA SETYANINGSIH. (2019). Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas. Biodiversitas Journal of Biological Diversity, 20(10). https://doi.org/10.13057/biodiv/d201016
Etesami, H., Alikhani, H. A., & Hosseini, H. M. (2015). Indole-3-acetic acid (IAA) production trait, a useful screening to select endophytic and rhizosphere competent bacteria for rice growth promoting agents. MethodsX, 2, 72–78. https://doi.org/10.1016/j.mex.2015.02.008
Fitri, R. (2018). ISOLASI DAN KARAKTERISASI Plant Growth Promoting Rhizobacteria (PGPR) Dari Rizosfer Tanaman Durian (Durio zibethinus Murr.) Skripsi: Universitas Islam Negeri Sultan Syarif Kasim Riau.
Fu, S.-F., Wei, J.-Y., Chen, H.-W., Liu, Y.-Y., Lu, H.-Y., & Chou, J.-Y. (2015). Indole-3-acetic acid: A widespread physiological code in interactions of fungi with other organisms. Plant Signaling & Behavior, 10(8), e1048052. https://doi.org/10.1080/15592324.2015.1048052
Gang, S., Sharma, S., Saraf, M., Buck, M., & Schumacher, J. (2019). Analysis of indole-3-acetic acid (IAA) production in Klebsiella by LC-MS/MS and the Salkowski method. Bio-Protocol, 9(9), e3230–e3230.
Hashem, A., Tabassum, B., & Fathi Abd_Allah, E. (2019). Bacillus subtilis: A plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi Journal of Biological Sciences, 26(6), 1291–1297. https://doi.org/10.1016/j.sjbs.2019.05.004
Hayat, R., Ali, S., Amara, U., Khalid, R., & Ahmed, I. (2010). Soil beneficial bacteria and their role in plant growth promotion: a review. Annals of Microbiology, 60, 579–598.
Hengkengbala, S. I., Lintang, R. A., Sumilat, D. A., Mangindaan, R. E., Ginting, E. L., & Tumembouw, S. (2021). Karakteristik Morfologi Dan Aktivitas Enzim Protease Bakteri Simbion Nudibranch. Jurnal Pesisir Dan Laut Tropis, 9(3), 83. https://doi.org/10.35800/jplt.9.3.2021.36672
Hutapea, V. H. (2023). Isolasi dan Karakterisasi Bakteri Penghasil Iaa (Indole Acetic Acid) dari Rhizosfer Tanaman Karet (Hevea brasiliensis Mull. Arg.) [Skripsi]. Universitas Sebelas Maret.
Jacoby, R., Peukert, M., Succurro, A., Koprivova, A., & Kopriva, S. (2017). The Role of Soil Microorganisms in Plant Mineral Nutrition—Current Knowledge and Future Directions. Frontiers in Plant Science, 8. https://doi.org/10.3389/fpls.2017.01617
Jeyanthi, V., & Kanimozhi, S. (2018). Plant growth promoting rhizobacteria (PGPR)-prospective and mechanisms: a review. J Pure Appl Microbiol, 12(2), 733–749.
Kandel, S., Joubert, P., & Doty, S. (2017). Bacterial Endophyte Colonization and Distribution within Plants. Microorganisms, 5(4), 77. https://doi.org/10.3390/microorganisms5040077
Kochar, M., Upadhyay, A., arun, vaishnavi, & Srivastava, S. (2013). Bacterial Biosynthesis of Indole-3-Acetic Acid: Signal Messenger Service. In Molecular Microbial Ecology of the Rhizosphere (Vol. 1). https://doi.org/10.1002/9781118297674.ch29
Laskow, S. (2018). There’s a bunch of gross stuff, besides human bodies, hiding under graveyards, URL: https://www.atlasobscura.com/articles/cemetery-soil-human-remains (accessed on 23 March 2023).
Lewis, K. (2000). Programmed Death in Bacteria. Microbiology and Molecular Biology Reviews, 64(3), 503–514. https://doi.org/10.1128/MMBR.64.3.503-514.2000
Lubis, A. F. (2021). Keanekaragaman Organisme Tanah pada Kelapa Sawit di Daerah Endemik Ganoderma boninense Pat. di Kebun Tanjung Beringin, Langkat [Tesis]. Universitas Sumatera Utara.
Lumombo, D. T. (2017). Kajian Yuridis Peraturan Pemerintah Republik Indonesia Nomor 9 Tahun 1987 Tentang Penyediaan Dan Penggunaan Tanah Untuk Keperluan Tempat Pemakaman. Lex Crimen, 6(8).
Nascimento, F. X., Rossi, M. J., & Glick, B. R. (2016). Role of ACC Deaminase in Stress Control of Leguminous Plants. In Plant Growth Promoting Actinobacteria (pp. 179–192). Springer Singapore. https://doi.org/10.1007/978-981-10-0707-1_11
Nuraini, A. N., Aisyah, & Ramdan, E. P. (2020). Seleksi Bakteri Rhizosfer Tanaman Rambutan Sebagai Agens Biokontrol Penyakit Antraknosa Pada Cabai (Capsicum annum L.). Jurnal Pertanian Presisi (Journal of Precision Agriculture), 4(2), 100–112. https://doi.org/10.35760/jpp.2020.v4i2.2999
Orozco-Mosqueda, Ma. del C., Glick, B. R., & Santoyo, G. (2020). ACC deaminase in plant growth-promoting bacteria (PGPB): An efficient mechanism to counter salt stress in crops. Microbiological Research, 235, 126439. https://doi.org/10.1016/j.micres.2020.126439
Rahayu, T., Purwestri, Y. A., Subandiyah, S., & Widianto, D. (2021). Potensi Bakteri Endofit Asal Tanaman Pisang Klutuk (Musa balbisiana Colla) Sebagai Pendukung Pertumbuhan Tanaman. Al-Kauniyah: Jurnal Biologi, 14(2), 313–324.
Ristiari, N. P. N., Julyasih, K. S. M., & Suryanti, I. A. P. (2019). Isolasi dan identifikasi jamur mikroskopis pada rizosfer tanaman jeruk siam (Citrus nobilis Lour.) di Kecamatan Kintamani, Bali. Jurnal Pendidikan Biologi Undiksha, 6(1), 10–19.
Santoyo, G., Moreno-Hagelsieb, G., del Carmen Orozco-Mosqueda, Ma., & Glick, B. R. (2016). Plant growth-promoting bacterial endophytes. Microbiological Research, 183, 92–99. https://doi.org/10.1016/j.micres.2015.11.008
Sudewi, S., Ala, A., Patandjengi, B., Bdr, M. F., & Rahim, A. (2020). Scereening of Plant Growth Promotion Rhizobacteria (PGPR) to increase local aromatic rice plant growth. Int. J. Pharm. Res, 13.
Suliasih, & Widawati, S. (2020). Isolation of Indole Acetic Acid (IAA) producing Bacillus siamensis from peat and optimization of the culture conditions for maximum IAA production. IOP Conference Series: Earth and Environmental Science, 572(1), 012025.
Vejan, P., Abdullah, R., Khadiran, T., Ismail, S., & Nasrulhaq Boyce, A. (2016). Role of Plant Growth Promoting Rhizobacteria in Agricultural Sustainability—A Review. Molecules, 21(5), 573. https://doi.org/10.3390/molecules21050573
Walida, H., Harahap, F. S., Hasibuan, M., & Yanti, F. F. (2019). Isolasi dan identifikasi bakteri penghasil IAA dan pelarut fosfat dari rhizosfer tanaman kelapa sawit. BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan), 6(1), 1–7.
Wallenstein, M. (2017, July 28). To restore our soils, feed the microbes, URL: https://theconversation.com/to-restore-our-soils-feed-the-microbes-79616 (accessed on 23 March 2023).
Xu, L., & Liu, Y. (2014). Protein secretion systems in bacterial pathogens. Frontiers in Biology, 9(6), 437–447. https://doi.org/10.1007/s11515-014-1333-z
Zarei, T., Moradi, A., Kazemeini, S. A., Akhgar, A., & Rahi, A. A. (2020). The role of ACC deaminase producing bacteria in improving sweet corn (Zea mays L. var saccharata) productivity under limited availability of irrigation water. Scientific Reports, 10(1), 20361. https://doi.org/10.1038/s41598-020-77305-6
Authors
Putra, S. S., Rahayu, T., & Tyastuti, E. M. (2023). Isolation and Characterization of Cambodian Tree Rhizospheric Bacteria (Plumeria acuminata) at Pracimaloyo TPU as a producer of IAA. BIOEDUSCIENCE, 7(1), 15–23. https://doi.org/10.22236/jbes/7111375
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