The Effect of Packaging and Encapsulation Temperature of Red Chili Seeds with Trichoderma sp. on Viability and Inhibition of Fusarium sp.

Dewanggie Sasmita Ratu, Hery Nirwanto, Tri Mujoko
https://doi.org/10.22236/jbes/18020

Article Sidebar

Issue
Vol. 9 No. 3 (2025): BIOEDUSCIENCE
Submitted
10 January 2025
Published
30 November 2025
Keywords:
    Biological Agent, Encapsulation, Fusarium sp., Packaging, Storage Temperature, Trichoderma sp. Agen hayati, Enkapsulasi, Fusarium sp., Kemasan, Suhu Penyimpanan, Trichoderma sp.
PDF

Abstract

Background: Fusarium sp. is one of the main threats to chili cultivation, thereby reducing its economic value. Therefore, the use of biological microorganisms, such as Trichoderma sp., is an environmentally friendly alternative for controlling Fusarium sp. Application techniques for Trichoderma sp. biological agents include seed coating or encapsulation. However, ensuring the quality and effectiveness of encapsulated products during storage and distribution remains a concern. Thus, this study aims to examine the effects of packaging type and storage temperature on the viability and efficacy of Trichoderma sp. biological agents encapsulated in red chili seeds (Capsicum annuum L.) for inhibiting Fusarium sp. Methods: This study applied a completely randomized design (CRD) factorial with two factors, namely packaging material (aluminum foil and plastic) and storage temperature (5°C, 28°C, and 36°C), resulting in 7 treatments with three replications plus a control, resulting in a total of 21 experimental units. Results: Aluminum foil packaging stored at 28°C showed the highest viability of Trichoderma sp. and the most effective antagonistic activity against Fusarium sp. Conclusion: These results provide a sustainable, environmentally friendly solution for biological-agent-based seed storage.

Full text article

Generated from XML file

References

Anis Nur Amalia dan Adelia Elviantari. 2023. Eksplorasi dan isolasi Trichoderma spp. pada rizosfer kopi robusta dibeberapa Kecamatan Sumbawa. J. Life Science and Technology. Vol.1, No.1: 13-21. Universitas Teknologi Sumbawa.
Badan Pusat Statistika. 2023. Produksi Tanaman Sayuran Cabai Besar, Cabai Rawit, Cabai Keriting di Provinsi Jawa Timur. Jawa Timur : BPS. https://jatim.bps.go.id/statictable/2023/03/16/2536/-produksi-tanaman-say uran-cabai-besar-cabai-rawit-cabai-keriting-menurut-kabupaten-kotadan-je nis-tanaman-di-provinsi-jawa-timur-kuintal-2021-dan2022.html.
Dharmaputra, O. S., Gunawan, A. W., Wulandari, R., & Basuki, T. 1999. Cendawan kontaminan dominan pada bedengan jamur merang dan interaksinya dengan jamur merang secara in-vitro. Jurnal Mikrobiologi Indonesia, 4(1), 14–18.
Hasanah, U., N. M. L. Ernawati, dan I. M Sudantha. 2016. Uji campuran Tricoderma spp. dengan ekstrak fungisida (kunyit dan daun sirih) terhadap jamur Fusarium oxysporum f.sp. capcaisi penyebab penyakit layu pada tanaman cabai. Ekosains, 9 (2) : 91-100.
Kanakdande, D., Bhosale, R., and Singhal, R.S. 2007. Stability of cumin, oleoresin microencapsulate in different combination of gum arab, maltodextrin and modified starch as wall material. Carbohydrate Polymers, 61 :95-102.
Kaur, A., et al. 2015. Cultural variability in Fusarium isolates. International Journal of Research Publication and Reviews, 5(8), 634-639.
Li, Y., & Shi, L. (2014). Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica. Cryobiology, 68(3): 446- 450.
Mahasri, G., Perdana, T. G. P., Kusnoto. (2019). Pengaruh Suhu Penyimpanan Terhadap Kerusakan Spora Myxobolus koi. Jurnal Ilmiah Perikanan dan Kelautan, 11(1) : 28-33.
Ningsih, H. U. S., Hastuti, D. L., & Listyorini, D. 2016. Kajian antagonis Trichoderma sp. terhadap Fusarium solani penyebab penyakit layu pada daun cabai rawit (Capsicum frutescens) secara in vitro. Proceeding Biology Education Conference, Vol. 13(1), 814–817.
Ou, S. H. 1985. Rice disease. Commonwealth mycological institute.
Patel, R., Sharma, N., & Kumar, S. 2022. Faktor lingkungan yang mempengaruhi viabilitas spora dalam penyimpanan. Applied Microbial Biotechnology , 60(4), 257-268.
Purwantisari, S. dan Rini B. H, 2009. Uji antagonisme jamur patogen phytophthora infestans penyebab penyakit busuk daun dan umbi tanaman kentang dengan menggunakan trichoderma spp. isolat lokal. BIOMA Vol. 11 (1), Hal. 24-32.
Putra, G. W. K., Yan, R., & Meitini, W. P. 2020. Eksplorasi dan identifikasi mikroba pada rhizosfer tanaman stroberi (Fragaria x ananassa Dutch.) di kawasan Pancasari Bedugul. Metamorfosa: Journal of Biological Sciences, 7(2), 62.
Ram, R., et al. (2021). Morphological variation in Fusarium oxysporum isolates. International Journal of Current Microbiology and Applied Sciences, 7(3), 1152-1162.
Ratnawati, Arfan, Kasman Jaya, Mufida. 2023. Kemampuan daya simpan dan daya tumbuh Trichoderma asperellum TR3 dalam berbagai kemasan. Jurnal Agrotech, 8 (1) 34-39.
Richy R. K. 2012. Pengaruh Macam BAhan Kemasan dan Kondisi Ruang Penyimpanan Terhadap Kulaitas Fisik dan Fisiologi Benih Kedelai (GLYCINE MAX.(L.) MERR.) Varietas Grobogan. SKRIPSI. UNIVERSITAS KRISTEN SATYA WACANA, Salatiga.
Sarah, H. M., 2023. Pengaruh Penyimpanan Terhadap Viabilitas dan Efektifitas Enkapsulasi Benih Selada Mengandung Trichoderma sp. dalam Menekan Penyakit Rebah Semai Rhizoctonia solani. Skripsi, Universitas Pembangunan Nasional Veteran Jawa Timur.
Sastrahidayat, I. R. 2011. Fitopatologi (ilmu penyakit tumbuhan). Malang: UB Press.
Shabrina, Q. A., dan Nugraheni, I. A. 2023. Optimasi aktivitas antibakteri metabolit sekunder dari bakteri endofit asal tanaman ciplukan (Physalis angulata L.). Yogyakarta. Vol 1, Hal: 327-337.
Suanda, I. Wayan. 2019. Karakterisasi Morfologis Trichoderma Sp. Isolat Jb Dan Daya Hambatnya Terhadap Jamur Fusarium Sp. Penyebab Penyakit Layu Dan Jamur Akar Putih Pada Beberapa Tanaman. 10, 99–112.
Sulistiyono D. F. (2015). Karakteristik fisiologi empat antagonis isolate Trichoderma sp. sebagai agensia hayati. Universitas Nusa Bangsa. Jurnal Sains Natural. 5(1) : 24-29.
Yetti, E., Irfandri, Aisyah, S., Dimas. W., Alfin. R., Maulana, M. 2024. Identifikasimmorfologi jamur penyebab penyakit busuk buah kakao dan uji daya hambat Bacillus spp. terhadap jamur tersebut secara in vitro. Jurnal Budidaya Pertanian. Vol. 20(1): 83-89
Yulia, E., H. S. Muhadam, F. Widiantini, dan W. Kurniawan. 2019. Perlakuan benih ekstrak Anredera cordifolia menekan kejadian penyakit antraknosa benih cabai terinfeksi Colletotricum acutatum. Agrikultura, 30(2):75.
Widiyanti, A., Jogeneis Patty, Gratiana N.C Tuhumury. 2022. Eksplorasi dan identifikasi jamur antagonis pada rizosfer tanaman cengkih (Syzygium aromaticum L.) di pulau Ambon. AGROLOGIA. 11(2):168-186.
Wuryandari, Y. 2004. Daya tahan hidup Pseudomonas putida Strain pf-20 dalam beberapa macam inokulum. Perlindungan Tanaman Indonesia, 10(1):33-41.

Authors

Dewanggie Sasmita Ratu
Departement of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 6029, Indonesia
Hery Nirwanto
Departement of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 6029, Indonesia
herry_n@upnjatim.ac.id (Primary Contact)
Tri Mujoko
National Development University “Veteran” East Java
Ratu, D. S., Nirwanto, H., & Mujoko, T. (2025). The Effect of Packaging and Encapsulation Temperature of Red Chili Seeds with Trichoderma sp. on Viability and Inhibition of Fusarium sp . BIOEDUSCIENCE, 9(3), 329–339. https://doi.org/10.22236/jbes/18020
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

Article Details