Test of the Effectiveness of Red Betel Leaves (Piper crocatum) on Alloxan-Induced Reduction of Blood Sugar Levels in Male White Mice (Mus musculus)
Abstract
Background: Hyperglycemia is when blood sugar levels increase excessively from normal sugar levels. High blood sugar levels in the body that exceed normal limits occur when the random blood sugar test value is ≥200 mg/dl and fasting blood sugar is ≥126 mg/dl. One natural ingredient known to lower blood sugar levels is red betel leaf (Piper crocatum). Methods: The method used in this research is the extraction and fractionation of n-hexane, ethyl acetate fraction, n-butanol fraction, and residual water fraction. Results: The results of research that has been carried out by giving fractionated n-butanol is effective in reducing blood sugar levels in male white mice (Mus musculus) that have been induced by alloxan. Conclusions: The positive group was obtained, and the results of administering the n-butanol fraction at a dose of 100 mg/KgBW affected reducing the blood sugar levels of male mice (Mus musculus) which had been induced by alloxan on days 2, 4, and 6, which decreased the most.
Full text article
Generated from XML file
References
Alza, Y. (2013). Hubungan Hiperglikemia Dengan Kadar Glyceraldehyde-3-Phosphate Dehydrogenase (Gapdh) Pada Penderita Diabetes Mellitus Tipe 2. Photon: Jurnal Sain Dan Kesehatan, 4(1), 19–26. https://doi.org/10.37859/jp.v4i1.165
Astuti, I. P., & Munawaroh, E. (2011). Karakteristik Mofrologi Daun Sirih Merah: Piper crocatum Ruitz & Pav dan Piper porphyrophyllum N.E.Br. Koleksi Kebun Raya Bogor. Berk. Penel. Hayati Edisi Khusus, 7A, 83–85.
Bohari, B., Nuryani, N., Abdullah, R., Amaliah, L., & Hafid, F. (2021). Hubungan aktivitas fisik dan obesitas sentral dengan hiperglikemia wanita dewasa: Cross-sectional study. AcTion: Aceh Nutrition Journal, 6(2), 199. https://doi.org/10.30867/action.v6i2.587
Chen, R., Ovbiagele, B., & Feng, W. (2018). Diabetes and Stroke: Epidemiology, Pathophysiology, Pharmaceuticals and Outcomes. Physiology & Behavior, 176(1), 139–148. https://doi.org/10.1016/j.amjms.2016.01.011.Diabetes
Dechayont, B., Phuaklee, P., Chunthorng-Orn, J., Juckmeta, T., Prajuabjinda, O., & Jiraratsatit, K. (2021). Antibacterial, Anti-inflammatory and Antioxidant Activities of Mahanintangtong and its Constituent Herbs, a Formula Used in Thai Traditional Medicine for Treating Pharyngitis. BMC Complementary Medicine and Therapies, 21(1), 1–12. https://doi.org/10.1186/s12906-021-03274-6
Deng, Y., He, K., Ye, X., Chen, X., Huang, J., Li, X., Yuan, L., Jin, Y., Jin, Q., & Li, P. (2012). Saponin rich fractions from Polygonatum odoratum (Mill.) Druce with more potential hypoglycemic effects. Journal of Ethnopharmacology, 141(1), 228–233. https://doi.org/10.1016/j.jep.2012.02.023
Hermes, L., Römermann, J., Cramer, B., & Esselen, M. (2021). Quantitative Analysis of β-Asarone Derivatives in Acorus calamus and Herbal Food Products by HPLC-MS/MS. Journal of Agricultural and Food Chemistry, 69(2), 776–782. https://doi.org/10.1021/acs.jafc.0c05513
Herwanto, M. E., & Rumampuk, J. F. (2016). Pengaruh Aktivitas Fisik Terhadap Kadar Gula Darah Pada Pria Dewasa. 4, 0–5.
Idris, H., Hasyim, H., & Utama, F. (2017). Analysis of Diabetes Mellitus Determinants in Indonesia: A Study from the Indonesian Basic Health Research 2013. Acta Medica Indonesiana, 49(4), 291–298.
Khan, M. A. A., & Islam, M. T. (2012). Analgesic and Cytotoxic Activity of Acorus calamus L., Kigelia pinnata L., Mangifera indica L. and Tabernaemontana divaricata L. Journal of Pharmacy and Bioallied Sciences, 4(2), 149–154. https://doi.org/10.4103/0975-7406.94820
Kohar, I., & Kartini. (2016). Media Pharinaceutica lndonesiana. In Media Pharinaceutica lndonesiana (Vol. 1, Issue 2).
Liu, X., Kim, J. K., Li, Y., Li, J., Liu, F., & Chen, X. (2005). Tannic acid stimulates glucose transport and inhibits adipocyte differentiation in 3T3-L1 cells. Journal of Nutrition, 135(2), 165–171. https://doi.org/10.1093/jn/135.2.165
Rezeki, S., Chismirina, S., & Iski, A. (2017). Pengaruh Ekstrak Daun Sirih Merah (Piper crocatum) terhadap Pertumbuhan Candida albicans. JDS (Journal of Syiah Kuala Dentistry Society), 2(1), 52–62.
Rusdi, M. S. (2020). Hipoglikemia Pada Pasien Diabetes Melitus. Journal Syifa Sciences and Clinical Research, 2(September), 83–90.
Setyawati, A., Sri, M., Wahyuningsih, H., Aris, D., Nugrahaningsih, A., Effendy, C., & Ibeneme, S. (2023). Piper Crocatum Ruiz & Pav as A Commonly Used Typically Medicinal Plant From Indonesia: What Do We Actually Know About It? Scoping Review. Indonesian Contemporary Nursing Journal, 7(2), 61–78.
Sinata, N., & Arifin, H. (2016). Antidiabetic Effect of Air Leaf Fraction of Karamunting (Rhodomyrtus tomentosa (Ait.) Hassk.) in Diabetic Mice. Jurnal Sains Farmasi & Klinis, 3(1), 72–78.
Subiyono, Martsiningsih, M. A., & Gabrela, D. (2016). Gambaran Kadar Glukosa Darah Metode GOD-PAP (Glucose Oxsidase – Peroxidase Aminoantypirin) Sampel Serum dan Plasma EDTA (Ethylen Diamin Terta Acetat). Jurnal Teknologi Laboratorium, 5(1), 45–48.
Triandita, N., R. Zakaria, F., Prangdimurti, E., & Eska Putri, N. (2016). Perbaikan Status Antioksidan Penderita Diabetes Tipe 2 Dengan Tahu Kedelai Hitam Kaya Serat. Jurnal Teknologi Dan Industri Pangan, 27(2), 123–130. https://doi.org/10.6066/jtip.2016.27.2.123
Yin, X., Zhang, Y., Wu, H., Zhu, X., Zheng, X., Jiang, S., Zhuo, H., Shen, J., Li, L., & Qiu, J. (2004). Protective effects of Astragalus saponin I on early stage of diabetic nephropathy in rats. Journal of Pharmacological Sciences, 95(2), 256–266. https://doi.org/10.1254/jphs.FP0030597
Astuti, I. P., & Munawaroh, E. (2011). Karakteristik Mofrologi Daun Sirih Merah: Piper crocatum Ruitz & Pav dan Piper porphyrophyllum N.E.Br. Koleksi Kebun Raya Bogor. Berk. Penel. Hayati Edisi Khusus, 7A, 83–85.
Bohari, B., Nuryani, N., Abdullah, R., Amaliah, L., & Hafid, F. (2021). Hubungan aktivitas fisik dan obesitas sentral dengan hiperglikemia wanita dewasa: Cross-sectional study. AcTion: Aceh Nutrition Journal, 6(2), 199. https://doi.org/10.30867/action.v6i2.587
Chen, R., Ovbiagele, B., & Feng, W. (2018). Diabetes and Stroke: Epidemiology, Pathophysiology, Pharmaceuticals and Outcomes. Physiology & Behavior, 176(1), 139–148. https://doi.org/10.1016/j.amjms.2016.01.011.Diabetes
Dechayont, B., Phuaklee, P., Chunthorng-Orn, J., Juckmeta, T., Prajuabjinda, O., & Jiraratsatit, K. (2021). Antibacterial, Anti-inflammatory and Antioxidant Activities of Mahanintangtong and its Constituent Herbs, a Formula Used in Thai Traditional Medicine for Treating Pharyngitis. BMC Complementary Medicine and Therapies, 21(1), 1–12. https://doi.org/10.1186/s12906-021-03274-6
Deng, Y., He, K., Ye, X., Chen, X., Huang, J., Li, X., Yuan, L., Jin, Y., Jin, Q., & Li, P. (2012). Saponin rich fractions from Polygonatum odoratum (Mill.) Druce with more potential hypoglycemic effects. Journal of Ethnopharmacology, 141(1), 228–233. https://doi.org/10.1016/j.jep.2012.02.023
Hermes, L., Römermann, J., Cramer, B., & Esselen, M. (2021). Quantitative Analysis of β-Asarone Derivatives in Acorus calamus and Herbal Food Products by HPLC-MS/MS. Journal of Agricultural and Food Chemistry, 69(2), 776–782. https://doi.org/10.1021/acs.jafc.0c05513
Herwanto, M. E., & Rumampuk, J. F. (2016). Pengaruh Aktivitas Fisik Terhadap Kadar Gula Darah Pada Pria Dewasa. 4, 0–5.
Idris, H., Hasyim, H., & Utama, F. (2017). Analysis of Diabetes Mellitus Determinants in Indonesia: A Study from the Indonesian Basic Health Research 2013. Acta Medica Indonesiana, 49(4), 291–298.
Khan, M. A. A., & Islam, M. T. (2012). Analgesic and Cytotoxic Activity of Acorus calamus L., Kigelia pinnata L., Mangifera indica L. and Tabernaemontana divaricata L. Journal of Pharmacy and Bioallied Sciences, 4(2), 149–154. https://doi.org/10.4103/0975-7406.94820
Kohar, I., & Kartini. (2016). Media Pharinaceutica lndonesiana. In Media Pharinaceutica lndonesiana (Vol. 1, Issue 2).
Liu, X., Kim, J. K., Li, Y., Li, J., Liu, F., & Chen, X. (2005). Tannic acid stimulates glucose transport and inhibits adipocyte differentiation in 3T3-L1 cells. Journal of Nutrition, 135(2), 165–171. https://doi.org/10.1093/jn/135.2.165
Rezeki, S., Chismirina, S., & Iski, A. (2017). Pengaruh Ekstrak Daun Sirih Merah (Piper crocatum) terhadap Pertumbuhan Candida albicans. JDS (Journal of Syiah Kuala Dentistry Society), 2(1), 52–62.
Rusdi, M. S. (2020). Hipoglikemia Pada Pasien Diabetes Melitus. Journal Syifa Sciences and Clinical Research, 2(September), 83–90.
Setyawati, A., Sri, M., Wahyuningsih, H., Aris, D., Nugrahaningsih, A., Effendy, C., & Ibeneme, S. (2023). Piper Crocatum Ruiz & Pav as A Commonly Used Typically Medicinal Plant From Indonesia: What Do We Actually Know About It? Scoping Review. Indonesian Contemporary Nursing Journal, 7(2), 61–78.
Sinata, N., & Arifin, H. (2016). Antidiabetic Effect of Air Leaf Fraction of Karamunting (Rhodomyrtus tomentosa (Ait.) Hassk.) in Diabetic Mice. Jurnal Sains Farmasi & Klinis, 3(1), 72–78.
Subiyono, Martsiningsih, M. A., & Gabrela, D. (2016). Gambaran Kadar Glukosa Darah Metode GOD-PAP (Glucose Oxsidase – Peroxidase Aminoantypirin) Sampel Serum dan Plasma EDTA (Ethylen Diamin Terta Acetat). Jurnal Teknologi Laboratorium, 5(1), 45–48.
Triandita, N., R. Zakaria, F., Prangdimurti, E., & Eska Putri, N. (2016). Perbaikan Status Antioksidan Penderita Diabetes Tipe 2 Dengan Tahu Kedelai Hitam Kaya Serat. Jurnal Teknologi Dan Industri Pangan, 27(2), 123–130. https://doi.org/10.6066/jtip.2016.27.2.123
Yin, X., Zhang, Y., Wu, H., Zhu, X., Zheng, X., Jiang, S., Zhuo, H., Shen, J., Li, L., & Qiu, J. (2004). Protective effects of Astragalus saponin I on early stage of diabetic nephropathy in rats. Journal of Pharmacological Sciences, 95(2), 256–266. https://doi.org/10.1254/jphs.FP0030597
Authors
Andriani, M., Putra, R. Y., & Rosyta, D. G. (2024). Test of the Effectiveness of Red Betel Leaves (Piper crocatum) on Alloxan-Induced Reduction of Blood Sugar Levels in Male White Mice (Mus musculus). BIOEDUSCIENCE, 8(2), 197–197. https://doi.org/10.22236/jbes/13320
This work is licensed under a Creative Commons Attribution 4.0 International License.