The Effectiveness of Nutrient Culture Solutions with Agar Addition as An Evaluation Media of Rice Under Iron Toxicity Conditions
Article Sidebar
-
Agar solution, Leaf bronzing, Rice Agar 0.2%, Bronzing Daun, HSYA, pH
Abstract
Background: Evaluation of the tolerance level of rice to iron (Fe) toxicity stress can be done using a hydroponic system in a nutrient culture solution under a controlled condition. This study aimed to obtain a nutrient culture solution that effective as a medium for evaluating the response of rice under Fe toxicity stress condition. Methods: This experiment was carried out by comparing the effectiveness of three kinds of nutrient culture media, namely Yoshida's Half-Strength solution (HSY), Yoshida's Half-Strength + 0.2% agar solution (HSYA), and Yoshida's Full-Strength + 0.2% agar solution (FSYA) using two rice genotypes, Inpara 5 (sensitive to Fe toxicity) and Mahsuri (tolerant to Fe toxicity). Leaf bronzing level, plant dry weight, and pH of nutrient culture media were observed in this experiment. Results: The results showed that the stress response as represented by the bronzing score in Inpara 5 leaves was known to be higher than that of Mahsuri in the three nutrient culture media. The decrease of root and shoot dry weight in Inpara 5 was higher than that of Mahsuri. In addition, the decrease in the pH of nutrient culture solution media without an agar addition (HSY) occurred faster than the media with the agar addition (HSYA and FSYA). Conclusion: The HSYA and FSYA media exhibited a similar pattern of pH declining but causing significant differences in growth responses between Inpara 5 and Mashuri indicating the HSYA medium is considered more efficient compared to the FSYA medium because it only requires a smaller amount of agar.
Full text article
References
Becker, M., & Asch, F. (2005). Iron toxicity in rice: conditions and management concepts. J Plant Nutr Soil Sci., 168, :558-573.
Brumbarova, T., & Bauer, P. (2008). Plant Membrane and Vacuolar Transporters. CAB International.
Devi, A. G., Yadav, G. S., Devi, H. L., & Ngachan, S. V. (2016). Effect of acute iron toxicity on key antioxidative enzymes in contrasting rice (Oryza sativa L.) cultivars of North-East India. IJBSM, 7(3), 388–392.
Dhiman, S. S., Selvaraj, C., Li, J., Singh, R., Zhao, X., Kim, D. W., Kim, J. Y., Kang, Y. C., & Lee., J. K. (2016). Phytoremediation of metal-contaminated soils by the hyperaccumulator canola (Brassica napus L.) and the use of its biomass for ethanol production. Fuel, 183, 107-114.
Elec, V., Quimio, C. A., Mendoza, R., Sajise, A. G. C., Beebout, S. E. J., Gregorio, G. B., & Singh, R. K. (2013). Maintaining elevated Fe2+ concentration in solution culture for the development of a rapid and repeatable screening technique for iron toxicity tolerance in rice (Oryza sativa L.). Plant Soil, 372(1), 253–264.
Engel, K. (2009). Efficiency of adaptation mechanisms of rice to diverse conditions of iron toxicity.
Engel, K., Asch, F., & Becker, M. (2012). Classification of rice genotypes based on their mechanisms of adaptation to iron toxicity. J Plant Nutr Soil Sci., 175, 871–881.
Jeong, J., & Connolly, E. L. (2009). Iron uptake mechanisms in plants: Functions of the FRO family of ferric reductases. Plant Sci, 176, 709–714.
Kabir, A. H., Begum, M. C., Haque, A., Amin, R., Swaraz, A. M., Haider, S. A., Paul, N. K., & Hossain, M. M. (2016). Genetic variation in Fe toxicity tolerance is associated with the regulation of translocation and chelation of iron along with antioxidant defence in shoots of rice. Funct Plant Biol, 43(11), A-L.
Kampfenkel, K., Montagu, M. V., & Inze, D. (1995). Effects of iron excess on Nicotiana plumbaginifolia plants: implication to oxidation stress. Plant Physiol, 107, 725–735.
Khabaz-Saberi, H., Rengel, Z., Wilson, R., & Setter, T. . (2010). Variation for tolerance to high concentration of ferrous iron (Fe2+) in Australian hexaploid wheat. Euphytica, 172, 275–283.
Li, G., Kronzucker, H. J., & Shi, W. (2016). The response of the root apex in plant adaptation to iron heterogeneity in soil. Front Plant Sci, 7, 1–7.
Marschner, H. (1995). Mineral Nutrition of Higher Plants. Academic Pr.
Matthus, E., Wu, L. B., Ueda, Y., Höller, S., Becker, M., & Frei, M. (2015). Loci, genes, and mechanisms associated with tolerance to ferrous iron toxicity in rice (Oryza sativa L.). Theor Appl Genet, 128(10), 2085-2098.
Muller, C., da Silveira Silveira, S. F., de Menezes Daloso, D., Mendes, G. C., Merchant, A., Kuki, K. N., Oliva, M. A., Loureiro, M. E., & Almeida, A. M. (2017). Echophysiological responses to excess iron in lowland and upland rice cultivars. Chemosphere., 189, 123–133.
Nugraha, Y., Ardie, S. W., Ghulamahdi, M., Suwarno, & Aswidinnoor, H. (2015). Nutrient culture media with agar is effective for early and rapid screening of iron toxicity tolerance in rice. J Crop Sci Biotech, 19(1), 61–70.
Quinet, M., Vromman, D., Clippe, A., Bertin, P., Lequeux, H., Dufey, I., Lutts, S., & Lefèvre, I. (2012). Combined transcriptomic and physiological approaches reveal strong differences between short- and long-term response of rice (Oryza sativa) to iron toxicity. Plant Cell Environ, 35(10), 1837–1859.
Shimizu, A., Guerta, C. Q., Gregorio, G. B., & Ikehashi, H. (2005). Improved mass screening of tolerance to iron toxicity in rice by lowering temperature of culture solution. J Plant Nut., 28, 1481–1493.
Stumm, W., & Lee, G. F. (1961). Oxygenation of ferrous iron. Ind Eng Chem, 53(2), 143–146.
Suryadi, D. (2012). Penapisan galur-galur padi (Oryza sativa L.) populasi RIL F7 hasil persilangan antara varietas IR64 dan Hawara Bunar terhadap cekaman Fe. IPB University.
Wang, Y., Frei, M., & Wissuwa, M. (2008). An agar nutrient solution technique as a screening tool for tolerance to zinc deficiency and iron toxicity in rice. Soil Sci Plant Nut, 54, 744–750.
Wu, L. B., Ueda, Y., Lai, S. K., & Frei, M. (2016). Shoot tolerance mechanisms to iron toxicity in rice (Oryza sativa L.). Plant Cell Environ, 40(4), 570–584.
Yoshida, S., Forno, D. A., Cock, J. H., & Gomez, K. A. (1976). Laboratory Manual for Physiological Studies of Rice. The International Rice Research Institute.
Zheng, S. J. (2010). Iron homeostasis and iron acquisition in plants: maintenance, functions and consequences. Ann Bot, 105, 799-800.
Authors
This work is licensed under a Creative Commons Attribution 4.0 International License.