Gonadal Differentiation of Nilem Fish (Osteochilus vittatus) Utilizing Temperature Treatment Gonadal Differentiation of Nilem Fish
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Submitted
8 October 2020
8 October 2020
Published
31 December 2020
31 December 2020
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Abstract
Background: Gonad differentiation in some teleostei is a critical phase of gonad development. Gonad differentiation in some fish such as the tilapia group is influenced by environmental factors, namely temperature. The aim of this study was to determine the differentiation process of Nilem fish gonads (Osteochilus vittatus) under the influence of temperature. Methods: One day after fertilization (days postfertilization/dpf) fish were exposed to different medium temperature ranges, namely: 28-29 oC, 30-31 oC, 32-33 oC, and room temperature as a control for 35 days. The percentage of fish survival was calculated on the 35th day by calculating the number of surviving fish divided by the total number of fish in 1 tank times 100%. Thirty fish in each treatment were measured for body length at day 10 dpf and day 35 dpf to determine fish growth. Six fish 35 dpf from each treatment were fixed in 70% alcohol for making histological preparations using the paraffin method, Haematoxylin-Eosin staining. Fish survival data growth data in the form of fish length were analyzed using Anova. Gonadal differentiation data were analyzed descriptively. Results: There was no significant difference in the growth of fish in all treatment and control groups. The average survival rate of fish in all treatment and control groups was 100%. Observation of the gonads showed that the gonads were not differentiated in all treatment and control groups. Conclusion: The room temperature range up to 33 oC supports the growth of Nilem fish with gonad development in the indifferent gonad stage.
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References
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Muchlisin, Z. A. (2014). Induced spawning of seurukan fish, Osteochilus vittatus (Pisces: Cyprinidae) using ovaprim, oxytocin and chicken pituitary gland extracts. November.
Nakamura, S., Kobayashi, K., Nishimura, T., & Tanaka, M. (2011). Ovarian germline stem cells in the teleost fish, medaka (Oryzias latipes). International Journal of Biological Sciences, 7(4), 403–409.
Netti Aryani, 2, & Ainul Mardiah, 2 Azrita and 2 Hafrijal Syandri 1. (2017). Fisheries and. December 2016. https://doi.org/10.3923/jfas.2017.29.35
Okuzawa, K., & Gen, K. (2013). General and Comparative Endocrinology High water temperature impairs ovarian activity and gene expression in the brain – pituitary – gonadal axis in female red seabream during the spawning season. General And Comparative Endocrinology, 194, 24–30. https://doi.org/10.1016/j.ygcen.2013.08.015
Pandit, N. P., Bhandari, R. K., Kobayashi, Y., & Nakamura, M. (2015). High Temperature-Induced Sterility In The Female Nile Tilapia, Oreochromis niloticus. Gen Comp Endocrinol, 213, 110–117.
Setyaningrum, N., Hidayah, H. A., & Article, H. (2017). Biosaintifika. 9(63), 257–264. https://doi.org/10.15294/biosaintifika.v9i2.9848
Syandri, H., Aryani, N., Mardiah., A., & Azrita. (2017). Effects of Enrichment Commercial Feed with Different Fat Sources on Survival and Growth of Bonylip Barb (Osteochilus vittatus Cyprinidae) Fingerlings. J.Fish.Aquat.Sci., 12(1), 29–35.
Tang, F. Nainoa Richardson, Audrey Albina, M.-C. C. and A. P.-G. (2020). cells-09-01103-v2(1).pdf.part. Mdpi, 9(5).
Tessema, M., Müller-Belecke, A., & Hörstgen-Schwark, G. (2006). Effect of rearing temperatures on the sex ratios of Oreochromis niloticus populations. Aquaculture, 258(1–4), 270–277. https://doi.org/10.1016/j.aquaculture.2006.04.041
Villamizar, N., Ribas, L., National, S., Piferrer, F., & National, S. (2014). Impact of Daily Thermocycles on Hatching Rhythms, Larval Performance and Impact of Daily Thermocycles on Hatching Rhythms, Larval Performance and Sex Differentiation of Zebrafish, (May). https://doi.org/10.1371/journal.pone.0052153
Wessels, S., & Hörstgen-Schwark, G. (2007). Selection experiments to increase the proportion of males in Nile tilapia (Oreochromis niloticus) by means of temperature treatment. Aquaculture, 272(SUPPL. 1). https://doi.org/10.1016/j.aquaculture.2007.08.009
Wessels, S., & Hörstgen-Schwark, G. (2011). Temperature dependent sex ratios in selected lines and crosses with a YY-male in Nile tilapia (Oreochromis niloticus). Aquaculture, 318(1–2), 79–84. https://doi.org/10.1016/j.aquaculture.2011.04.039
Wessels, S., Sharifi, R. A., Luehmann, L. M., Rueangsri, S., Krause, I., Pach, S., Hoerstgen-Schwark, G., & Knorr, C. (2014). Allelic variant in the anti-müllerian hormone gene leads to autosomal and temperature-dependent sex reversal in a selected nile tilapia line. PLoS ONE, 9(8). https://doi.org/10.1371/journal.pone.0104795
WIjayanti, et al. (2017). A Simple Paraffin Embedded Protocol For Fish Egg , Embryo , And Larvae. 4, 85–89.
Wilson, C. A., High, S. K., McCluskey, B. M., Amores, A., Yan, Y. L., Titus, T. A., Anderson, J. L., Batzel, P., Carvan, M. J., Schartl, M., & Postlethwait, J. H. (2014). Wild sex in zebrafish: Loss of the natural sex determinant in domesticated strains. In Genetics (Vol. 198, Issue 3). https://doi.org/10.1534/genetics.114.169284
Zulhardi Zulhardi*, Zainal A. Muchlisin, S. P. (2016). Effect of Zygote Age for Heat Shocking on The Successful of Gynogenesis of Pengaruh Umur Zigot Pada Saat Kejutan Panas Terhadap Keberhasilan Ginogenesis Ikan Seurukan ( Osteochilus Vittatus ) Effect of Zygote Age for Heat Shocking on The Successful of Gyn. December.
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Baroiller, J.-F., Chourrout, D., Fostier, A., & Jalabert, B. (1995). Temperature and sex chromosomes govern sex ratios of the mouthbrooding Cichlid fish (Oreochromis niloticus). Journal of Experimental Zoology, 273(3), 216–223. https://doi.org/10.1002/jez.1402730306
Baroiller, J. F., D'Cotta, H., & Saillant, E. (2009). Environmental effects on fish sex determination and differentiation. Sexual Development, 3(2–3), 118–135. https://doi.org/10.1159/000223077
D'Cotta, H., Fostier, a, Guiguen, Y., Govoroun, M., & Baroiller, J. F. (2001). Aromatase plays a key role during normal and temperature- induced sex differentiation of Tilapia Oreochromis niloticus. Molecular Reproduction and Development, 59(3), 265–276. https://doi.org/10.1002/mrd.1031
Eriani, K., Syahrin, A., Muchlisin, Z. A., & Article, H. (2017). Biosaintifika Effect of Temperature Shock on the Triploidization Success. 9(2), 298–303. https://doi.org/10.15294/biosaintifika.v9i2.8680
Habibah, A N, Hana, & Pratiwi, M. (2019). Perkembangan gonad benih ikan nilem yang dipelihara dalam temperatur berbeda. 1(November), 22–28.
Habibah, Aulidya N, & Wilting, J. (2017). Germline Development of Genetically Female Nile Tilapia (Oreochromis niloticus) Reared under. https://doi.org/10.1159/000479396
Herawati, H., Hasan, Z., & Rochima, E. (2019). Effect of Diffuser Aerator to Increase Growth of Nilem (Osteochilus vittatus) Cultivation With Different Density. March.
Kobayashi, Y., Nagahama, Y., & Nakamura, M. (2012). Diversity and plasticity of sex determination and differentiation in fishes. Sexual Development, 7(1–3), 115–125. https://doi.org/10.1159/000342009
Liew, W. C. and O. (2013). Zebrafish sex"¯: a complicated affair. https://doi.org/10.1093/bfgp/elt041
Martinez-Bengocheaa, 1, L. Dorettoa, 1, I.F. Rosaa, M.A. Oliveiraa, C. Silvaa, D.M.Z.A. Silvaa, G.R. Santosb, c, J.S.F. Santosb, c, M.M. Avelarb, c, L.V. Silvab, c, D. Lucianelli-Juniore, E.R.B. Souzab, C., R.C. Silvab, c, A.B. Stewartd, L.S.O. Nakaghib, c, F.N. Valentine,âŽ, R. H. N., Santos, J. S. F., Avelar, M. M., Silva, L. V, Souza, E. R. B., Silva, R. C., Stewart, A. B., Nakaghi, L. S. O., Valentin, F. N., & Nóbrega, R. H. (2020). Effects of 17 β -estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost , Astyanax altiparanae Comparative Biochemistry and Physiology , Part B E ff ects of 17 β -estradiol on early. Comparative Biochemistry and Physiology, Part B, 248–249(July), 110467. https://doi.org/10.1016/j.cbpb.2020.110467
Muchlisin, Z. A. (2014). Induced spawning of seurukan fish, Osteochilus vittatus (Pisces: Cyprinidae) using ovaprim, oxytocin and chicken pituitary gland extracts. November.
Nakamura, S., Kobayashi, K., Nishimura, T., & Tanaka, M. (2011). Ovarian germline stem cells in the teleost fish, medaka (Oryzias latipes). International Journal of Biological Sciences, 7(4), 403–409.
Netti Aryani, 2, & Ainul Mardiah, 2 Azrita and 2 Hafrijal Syandri 1. (2017). Fisheries and. December 2016. https://doi.org/10.3923/jfas.2017.29.35
Okuzawa, K., & Gen, K. (2013). General and Comparative Endocrinology High water temperature impairs ovarian activity and gene expression in the brain – pituitary – gonadal axis in female red seabream during the spawning season. General And Comparative Endocrinology, 194, 24–30. https://doi.org/10.1016/j.ygcen.2013.08.015
Pandit, N. P., Bhandari, R. K., Kobayashi, Y., & Nakamura, M. (2015). High Temperature-Induced Sterility In The Female Nile Tilapia, Oreochromis niloticus. Gen Comp Endocrinol, 213, 110–117.
Setyaningrum, N., Hidayah, H. A., & Article, H. (2017). Biosaintifika. 9(63), 257–264. https://doi.org/10.15294/biosaintifika.v9i2.9848
Syandri, H., Aryani, N., Mardiah., A., & Azrita. (2017). Effects of Enrichment Commercial Feed with Different Fat Sources on Survival and Growth of Bonylip Barb (Osteochilus vittatus Cyprinidae) Fingerlings. J.Fish.Aquat.Sci., 12(1), 29–35.
Tang, F. Nainoa Richardson, Audrey Albina, M.-C. C. and A. P.-G. (2020). cells-09-01103-v2(1).pdf.part. Mdpi, 9(5).
Tessema, M., Müller-Belecke, A., & Hörstgen-Schwark, G. (2006). Effect of rearing temperatures on the sex ratios of Oreochromis niloticus populations. Aquaculture, 258(1–4), 270–277. https://doi.org/10.1016/j.aquaculture.2006.04.041
Villamizar, N., Ribas, L., National, S., Piferrer, F., & National, S. (2014). Impact of Daily Thermocycles on Hatching Rhythms, Larval Performance and Impact of Daily Thermocycles on Hatching Rhythms, Larval Performance and Sex Differentiation of Zebrafish, (May). https://doi.org/10.1371/journal.pone.0052153
Wessels, S., & Hörstgen-Schwark, G. (2007). Selection experiments to increase the proportion of males in Nile tilapia (Oreochromis niloticus) by means of temperature treatment. Aquaculture, 272(SUPPL. 1). https://doi.org/10.1016/j.aquaculture.2007.08.009
Wessels, S., & Hörstgen-Schwark, G. (2011). Temperature dependent sex ratios in selected lines and crosses with a YY-male in Nile tilapia (Oreochromis niloticus). Aquaculture, 318(1–2), 79–84. https://doi.org/10.1016/j.aquaculture.2011.04.039
Wessels, S., Sharifi, R. A., Luehmann, L. M., Rueangsri, S., Krause, I., Pach, S., Hoerstgen-Schwark, G., & Knorr, C. (2014). Allelic variant in the anti-müllerian hormone gene leads to autosomal and temperature-dependent sex reversal in a selected nile tilapia line. PLoS ONE, 9(8). https://doi.org/10.1371/journal.pone.0104795
WIjayanti, et al. (2017). A Simple Paraffin Embedded Protocol For Fish Egg , Embryo , And Larvae. 4, 85–89.
Wilson, C. A., High, S. K., McCluskey, B. M., Amores, A., Yan, Y. L., Titus, T. A., Anderson, J. L., Batzel, P., Carvan, M. J., Schartl, M., & Postlethwait, J. H. (2014). Wild sex in zebrafish: Loss of the natural sex determinant in domesticated strains. In Genetics (Vol. 198, Issue 3). https://doi.org/10.1534/genetics.114.169284
Zulhardi Zulhardi*, Zainal A. Muchlisin, S. P. (2016). Effect of Zygote Age for Heat Shocking on The Successful of Gynogenesis of Pengaruh Umur Zigot Pada Saat Kejutan Panas Terhadap Keberhasilan Ginogenesis Ikan Seurukan ( Osteochilus Vittatus ) Effect of Zygote Age for Heat Shocking on The Successful of Gyn. December.
Authors
Habibah, A. N., Pertiwi, R. P., & Chasanah, T. (2020). Gonadal Differentiation of Nilem Fish (Osteochilus vittatus) Utilizing Temperature Treatment: Gonadal Differentiation of Nilem Fish. BIOEDUSCIENCE, 4(2), 143–147. https://doi.org/10.22236/j.bes/425704
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