Identification of Bacteria in Kawista Leaves (Limonia acidissima L.)
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Submitted
8 September 2020
Published
23 April 2021
Keywords:
-
identification, Isolation, Kawista, Limonia acidissima L
Abstract
Background: Bacteria are unicellular prokaryotic microorganisms which are abundant in nature. This study aims to isolate and identify bacteria present in the leaves of Kawista (Limonia acidissima L.). Method: The identification process was carried out in a microbiology analyzer with the help of the BD Phonix M50 identification tool. The study was conducted at the West Nusa Tenggara Province Testing and Calibration Laboratory for Health, using kawista (Limonia acidissima L.) leaves obtained from West Sumbawa Regency. Results: The results showed eight bacterial isolates were found including Bacillus sp, Bacillus megaterium, Staphylococcus sp, Staphylococcus epidermidis, Staphylococcus capitis, Streptococcus oralis, Leuconostoc mesenteroides spp. mesenteroides, and Shewanella putrefaciens. Conclusion: Diversity of types of bacteria can be studied through the process of identification. This study aims to isolate and identify bacteria found in kawista leaves. The identification method is carried out in a microbiology analyzer with the help of the BD Phonix M50 identification tool. Bacteria found on the leaves of kawista (Limonia acidissima L.) consist of a group of endophytic bacteria, lactic acid bacteria, metal reducing bacteria, and pathogenic bacteria.
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References
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Greco-Stewart, V. S., Ali, H., Kumaran, D., Kalab, M., Rood, I. G. H., de Korte, D., & Ramírez-Arcos, S. (2013). Biofilm Formation by Staphylococcus capitis Strains Isolated from Contaminated Platelet Concentrates. Journal of Medical Microbiology, 62(PART7), 1051–1059. DOI: 10.1099/jmm.0.050500-0
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Holzapfel, W. H., & Stiles, M. E. (1997). Lactic acid bacteria of foods and their current taxonomy. International Journal of Food Microbiology.
Johnsborg, O., Eldholm, V., Bjí¸rnstad, M. L., & Håvarstein, L. S. (2008). A Predatory Mechanism Dramatically Increases the Efficiency of Lateral Gene Transfer in Streptococcus Pneumoniae and Related Commensal Species. Molecular Microbiology, 69(1), 245–253.DOI: 10.1111/j.1365-2958.2008.06288.x
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Khashe, S., & Michael Janda, J. (1998). Biochemical and pathogenic properties of Shewanella alga and Shewanella putrefaciens. Journal of Clinical Microbiology, 36(3), 783–787. DOI: 10.1128/jcm.36.3.783-787.1998
Knoess, W., & Wiesner, J. (2019). The Globalization of Traditional Medicines: Perspectives Related to the European Union Regulatory Environment. Engineering, 5(1), 22–31. DOI: 10.1016/j.eng.2018.11.012
Kusumawati, N. (2012). Peranan Bakteri Asam Laktat Dalam Menghambat Listeria Monocytogenes Pada Bahan Pangan. Jurnal Teknologi Pangan Dan Gizi, 1(1), 14–28. DOI: 10.33508/JTPG.V1I1.77
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Mary, F., Nongkhlaw, W., & Joshi, S. R. (2014). Epiphytic and Endophytic Bacteria That Promote Growth of Ethnomedicinal Plants in The Subtropical Forests of Meghalaya, India. Revista de Biología Tropical, 62(December), 1295–1308.
Mekete, T., Hallmann, J., Kiewnick, S., & Sikora, R. (2009). Endophytic bacteria from ethiopian coffee plants and their potential to antagonise meloidogyne incognita. Nematology, 11(1), 117–127. DOI: 10.1163/156854108X398462
Namvar, A. E., Bastarahang, S., Abbasi, N., Ghehi, G. S., Farhadbakhtiarian, S., Arezi, P., ... Chermahin, S. G. (2014). Clinical characteristics of Staphylococcus epidermidis: a systematic review. GMS Hygiene and Infection Control, 9(3), DOI: 10.3205/dgkh000243
Nurdiana, Z., Ariyanti, N. S., & Hartana, A. (2016). Variasi Morfologi dan Pengelompokan Kawista (Limonia acidissima L.) di Jawa dan Kepulauan Sunda Kecil. Floribunda, 5(4), 144–156. DOI: 10.1007/978-94-007-4053-2_101
Panda, N., Patro, J., Jena, B. K., & Panda, P, K. (2013). Evaluation of Phytochemical and Anti-Microbial Activity of Ethanolic Extract of Limonia Acidissima L. Leaves. International Journal of Herbal Medicine, 1(1), 22–27.
Pujiati, P. (2019). Buku Aja Mikrobiologi Umum.
Puspita, F., Saputra, S. I., & Merini, D. J. (2019). Uji Beberapa Konsentrasi Bakteri Bacillus sp. Endofit untuk Meningkatkan Pertumbuhan Bibit Kakao (Theobroma cacao L.). Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 46(3), 322–327. DOI: 10.24831/jai.v46i3.16342
Badan POM RI (2012). Pedoman Teknologi Formulasi Sediaan Berbasis Ekstrak. Sumber Dokumen Resmi
Rodrigues, S., Brito, E. S. de, & Silva, E. de O. (2018). Wood Apple” Limonia acidissima. In Exotic Fruits. DOI: 10.1016/b978-0-12-803138-4.00060-5
Sambara, Jefrin., Yuliani, Ni Nyoman, Emeransia, M. Y. (2016). Pemanfaatan Tanaman Obat Tradisional Oleh Masyarakat Kelurahan Merdeka Kecamatan Kupang Timur 2016. Jurnal Info Kesehatan.
Santoyo, G., Moreno-Hagelsieb, G., del Carmen Orozco-Mosqueda, M., & Glick, B. R. (2016). Plant growth-promoting bacterial endophytes. Microbiological Research, 183, 92–99. DOI: 10.1016/j.micres.2015.11.008
Suhandono, S., Kusumawardhani, M. K., & Aditiawati, P. (2016). Isolation and Molecular Identification of Endophytic Bacteria From Rambutan Fruits (Nephelium lappaceum L.) Cultivar Binjai. HAYATI Journal of Biosciences, 23(1), 39–44. DOI: 10.1016/j.hjb.2016.01.005
Sumarsih, S. (2003). Mikrobiologi Dasar. Fakultas Pertanian UPN "Veteran” Yogyakarta. DOI: 10.1148/77.1.94
Tangapo, A. M., Astuti, D. I., & Aditiawati, P. (2018). Dynamics And Diversity Of Cultivable Rhizospheric And Endophytic Bacteria During The Growth Stages Of Cilembu Sweet Potato (Ipomoea batatas L. var. cilembu). Agriculture and Natural Resources, 52(4), 309–316. DOI: 10.1016/j.anres.2018.10.003
Turnip, M. (2018). Identifikasi Bakteri Anggota Enterobacteriaceae pada Makanan Tradisional Sotong Pangkong. 2(2), 6–12.
Venkateswaran, K., Moser, D. P., Dollhopf, M. E., Lies, D. P., Saffarini, D. A., MacGregor, B. J., ... Nealson, K. H. (1999). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. International Journal of Systematic Bacteriology. DOI: 10.1099/00207713-49-2-705
Vijayakumar, P., Punitha, K., & Banupriya, L. (2013). Drying characteristics and quality evaluation of wood apple (Limonia acidissima L.) fruit pulp powder. Int. J. Cur. Tr. Res.
Zhao, L. F., Xu, Y. J., & Lai, X. H. (2018). Antagonistic endophytic bacteria associated with nodules of soybean (Glycine max L.) and plant growth-promoting properties. Brazilian Journal of Microbiology. DOI: 10.1016/j.bjm.2017.06.007
Zinniel, D. K., Lambrecht, P., Harris, N. B., Feng, Z., Kuczmarski, D., Higley, P., ... Vidaver, A. K. (2002). Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. Applied and Environmental Microbiology. DOI: 10.1128/AEM.68.5.2198-2208.2002
Zulfa, N., Ariyanti, N. S., & Hartana, A. (2016). Variasi Morfologi Dan Pengelompokan Kawista. Floribunda. DOI: 10.1007/978-94-007-4053-2_101
Ameen, F., Reda, S. A., El-Shatoury, S. A., Riad, E. M., Enany, M. E., & Alarfaj, A. A. (2019). Prevalence of Antibiotic Resistant Mastitis Pathogens in Dairy Cows in Egypt and Potential Biological Control Agents Produced from Plant Endophytic Actinobacteria. Saudi Journal of Biological Sciences, 26(7), 1492–1498. DOI: 10.1016/j.sjbs.2019.09.008
Anjum, N., & Chandra, R. (2015). Endophytic Bacteria: Optimizaton of isolation Procedure from Various Medicinal Plants and Their Preliminary Characterization. Asian Journal of Pharmaceutical and Clinical Research, 8(4), 233–238.
Fatoba, O. S., Esezobor, D. E., Akanji, O. L., Fatoba, A. J., Macgregor, D., & Etubor, J. (2014). The Study of the Antimicrobial Properties of Selected Engineering Materials' Surfaces. Journal of Minerals and Materials Characterization and Engineering, 02(02), 78–87. DOI: 10.4236/jmmce.2014.22012
Greco-Stewart, V. S., Ali, H., Kumaran, D., Kalab, M., Rood, I. G. H., de Korte, D., & Ramírez-Arcos, S. (2013). Biofilm Formation by Staphylococcus capitis Strains Isolated from Contaminated Platelet Concentrates. Journal of Medical Microbiology, 62(PART7), 1051–1059. DOI: 10.1099/jmm.0.050500-0
Hidayat, H. (2015). Identifikasi Morfologi Dan Uji Aktivitas Antimikroba Terhadap Bakteri Escherichia Coli Dari Fermentasi Buah Markisa (Passiflora Sp.). Jurnal Eksakta, 15(1–2), 76–85. DOI: 10.20885/eksakta.vol15.iss1-2.art8
Holzapfel, W. H., & Stiles, M. E. (1997). Lactic acid bacteria of foods and their current taxonomy. International Journal of Food Microbiology.
Johnsborg, O., Eldholm, V., Bjí¸rnstad, M. L., & Håvarstein, L. S. (2008). A Predatory Mechanism Dramatically Increases the Efficiency of Lateral Gene Transfer in Streptococcus Pneumoniae and Related Commensal Species. Molecular Microbiology, 69(1), 245–253.DOI: 10.1111/j.1365-2958.2008.06288.x
Jung, J. Y., Lee, S. H., Kim, J. M., Park, M. S., Bae, J. W., Hahn, Y., ... Jeon, C. O. (2011). Metagenomic Analysis of Kimchi, a Traditional Korean Fermented Food. Applied and Environmental Microbiology, 77(7), 2264–2274. DOI: 10.1128/AEM.02157-10
Khashe, S., & Michael Janda, J. (1998). Biochemical and pathogenic properties of Shewanella alga and Shewanella putrefaciens. Journal of Clinical Microbiology, 36(3), 783–787. DOI: 10.1128/jcm.36.3.783-787.1998
Knoess, W., & Wiesner, J. (2019). The Globalization of Traditional Medicines: Perspectives Related to the European Union Regulatory Environment. Engineering, 5(1), 22–31. DOI: 10.1016/j.eng.2018.11.012
Kusumawati, N. (2012). Peranan Bakteri Asam Laktat Dalam Menghambat Listeria Monocytogenes Pada Bahan Pangan. Jurnal Teknologi Pangan Dan Gizi, 1(1), 14–28. DOI: 10.33508/JTPG.V1I1.77
López-Bucio, J., Campos-Cuevas, J. C., Hernández-Calderón, E., Velásquez-Becerra, C., Farías-Rodríguez, R., Macías-Rodríguez, L. I., & Valencia-Cantero, E. (2007). Bacillus megaterium rhizobacteria promote growth and alter root-system architecture through an auxin- and ethylene-independent signaling mechanism in Arabidopsis thaliana. Molecular Plant-Microbe Interactions, 20(2), 207–217. DOI: 10.1094/MPMI-20-2-0207
Mary, F., Nongkhlaw, W., & Joshi, S. R. (2014). Epiphytic and Endophytic Bacteria That Promote Growth of Ethnomedicinal Plants in The Subtropical Forests of Meghalaya, India. Revista de Biología Tropical, 62(December), 1295–1308.
Mekete, T., Hallmann, J., Kiewnick, S., & Sikora, R. (2009). Endophytic bacteria from ethiopian coffee plants and their potential to antagonise meloidogyne incognita. Nematology, 11(1), 117–127. DOI: 10.1163/156854108X398462
Namvar, A. E., Bastarahang, S., Abbasi, N., Ghehi, G. S., Farhadbakhtiarian, S., Arezi, P., ... Chermahin, S. G. (2014). Clinical characteristics of Staphylococcus epidermidis: a systematic review. GMS Hygiene and Infection Control, 9(3), DOI: 10.3205/dgkh000243
Nurdiana, Z., Ariyanti, N. S., & Hartana, A. (2016). Variasi Morfologi dan Pengelompokan Kawista (Limonia acidissima L.) di Jawa dan Kepulauan Sunda Kecil. Floribunda, 5(4), 144–156. DOI: 10.1007/978-94-007-4053-2_101
Panda, N., Patro, J., Jena, B. K., & Panda, P, K. (2013). Evaluation of Phytochemical and Anti-Microbial Activity of Ethanolic Extract of Limonia Acidissima L. Leaves. International Journal of Herbal Medicine, 1(1), 22–27.
Pujiati, P. (2019). Buku Aja Mikrobiologi Umum.
Puspita, F., Saputra, S. I., & Merini, D. J. (2019). Uji Beberapa Konsentrasi Bakteri Bacillus sp. Endofit untuk Meningkatkan Pertumbuhan Bibit Kakao (Theobroma cacao L.). Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 46(3), 322–327. DOI: 10.24831/jai.v46i3.16342
Badan POM RI (2012). Pedoman Teknologi Formulasi Sediaan Berbasis Ekstrak. Sumber Dokumen Resmi
Rodrigues, S., Brito, E. S. de, & Silva, E. de O. (2018). Wood Apple” Limonia acidissima. In Exotic Fruits. DOI: 10.1016/b978-0-12-803138-4.00060-5
Sambara, Jefrin., Yuliani, Ni Nyoman, Emeransia, M. Y. (2016). Pemanfaatan Tanaman Obat Tradisional Oleh Masyarakat Kelurahan Merdeka Kecamatan Kupang Timur 2016. Jurnal Info Kesehatan.
Santoyo, G., Moreno-Hagelsieb, G., del Carmen Orozco-Mosqueda, M., & Glick, B. R. (2016). Plant growth-promoting bacterial endophytes. Microbiological Research, 183, 92–99. DOI: 10.1016/j.micres.2015.11.008
Suhandono, S., Kusumawardhani, M. K., & Aditiawati, P. (2016). Isolation and Molecular Identification of Endophytic Bacteria From Rambutan Fruits (Nephelium lappaceum L.) Cultivar Binjai. HAYATI Journal of Biosciences, 23(1), 39–44. DOI: 10.1016/j.hjb.2016.01.005
Sumarsih, S. (2003). Mikrobiologi Dasar. Fakultas Pertanian UPN "Veteran” Yogyakarta. DOI: 10.1148/77.1.94
Tangapo, A. M., Astuti, D. I., & Aditiawati, P. (2018). Dynamics And Diversity Of Cultivable Rhizospheric And Endophytic Bacteria During The Growth Stages Of Cilembu Sweet Potato (Ipomoea batatas L. var. cilembu). Agriculture and Natural Resources, 52(4), 309–316. DOI: 10.1016/j.anres.2018.10.003
Turnip, M. (2018). Identifikasi Bakteri Anggota Enterobacteriaceae pada Makanan Tradisional Sotong Pangkong. 2(2), 6–12.
Venkateswaran, K., Moser, D. P., Dollhopf, M. E., Lies, D. P., Saffarini, D. A., MacGregor, B. J., ... Nealson, K. H. (1999). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. International Journal of Systematic Bacteriology. DOI: 10.1099/00207713-49-2-705
Vijayakumar, P., Punitha, K., & Banupriya, L. (2013). Drying characteristics and quality evaluation of wood apple (Limonia acidissima L.) fruit pulp powder. Int. J. Cur. Tr. Res.
Zhao, L. F., Xu, Y. J., & Lai, X. H. (2018). Antagonistic endophytic bacteria associated with nodules of soybean (Glycine max L.) and plant growth-promoting properties. Brazilian Journal of Microbiology. DOI: 10.1016/j.bjm.2017.06.007
Zinniel, D. K., Lambrecht, P., Harris, N. B., Feng, Z., Kuczmarski, D., Higley, P., ... Vidaver, A. K. (2002). Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. Applied and Environmental Microbiology. DOI: 10.1128/AEM.68.5.2198-2208.2002
Zulfa, N., Ariyanti, N. S., & Hartana, A. (2016). Variasi Morfologi Dan Pengelompokan Kawista. Floribunda. DOI: 10.1007/978-94-007-4053-2_101
Authors
Aulia, J., Purwati, N., & Umami, S. S. (2021). Identification of Bacteria in Kawista Leaves (Limonia acidissima L.). BIOEDUSCIENCE, 5(1). https://doi.org/10.22236/j.bes/515568
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