Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni
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Submitted
2 March 2020
Published
30 June 2020
Keywords:
-
Powdery mildew, resistance, meloni, Podosphera xanthii Embun tepung, ketahanan, meloni, Podosphera xanthii
Abstract
Background: A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. Meloni is a superior melon cultivar bred through a crossing between ♀ SL-3 and ♂ PI 371795, resulted by the Laboratory of Genetics and Breeding, Faculty of Biology, UGM. This study aimed to determine the level resistance of Meloni to powdery mildew infection and to identify the powdery mildew species that infected Meloni based on morphological characters. Methods: Meloni seeds were germinated and planted in the greenhouse of PIAT UGM. Powdery mildew spores were inoculated into the leaves after ±2 weeks of age. Leaf infected were scored using the gridline every 3 days for 6 weeks. Scoring results were converted to the diseases index score. Furthermore, powdery mildew species was identified using morphological characters. Results: Meloni had a tolerance level of resistance to the powdery mildew infection. Based on the morphological characters with fibrosin bodies, conidia ovoid-shape and the position of the germ tube in the lateral part of the conidia, powdery mildew that infected Meloni was expected as P. xanthii. Conclusions: Meloni can be expected as an alternative to superior melon seeds resistant to pest and disease infections especially powdery mildew.
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References
Aristya, G. R., & Daryono, B. S. (2013). Pengembangan dan Pewarisan Sifat Ketahanan Penyakit Powdery Mildew padaTanaman Melon (Cucumis melo L.) var. Tacapa. Jurnal Penelitian Dan Pengembangan Pemerintah Daerah DIY, 7(7), 47–52.
Aristya, G. Z., Suryanto, E., Kasiamdari, R. S., & Daryono, B. S. (2013). Seleksi Ketahanan Kultivar Tanaman Melon (Cucumis melo L.) Terhadap Jamur Tepung (Powdery Mildew) Isolat Ngawi. Jurnal Penelitian Dan Pengembangan, 8(8), 15–30.
Braun, U., & Cook, R. T. A. (2012). Taxonomic Manual of the Erypshiphales (Powdery Mildews). Utrecht: CBS KNAW fungal Biodiversity Center.
Cho, S. E., Choi, I. Y., Han, K. S., & Shin, H. D. (2017). First record of Podosphaera xanthii on Peperomia tetragona. Australasian Plant Diseases Notes, 12(1), 31.
Crowder, L. V. (1990). Genetika Tumbuhan. Yogyakarta: Gadjah Mada University.
Daryono, B. S., Aristya, G. R., & Kasiamdari, R. S. (2011). Development of random amplified polymorphism DNA markers linked to Powdery Mildew resistance gene in Melon. Indonesian Journal of Biotechnology, 16(2), 76–82. Retrieved from https://doi.org/10.22146/ijbiotech.7837
Daryono, B. S., E., V. A., & Al-Mughni, E. W. (2018). Phenotypic characters stability of melon (Cucumis melo L. Meloni cultivar). The Proceeding of the 2nd International Conference on Tropical Agriculture, 141–149. Yogyakarta.
Daryono, B. S., & Qurrohman, M. T. (2009). Pewarisan sifat ketahanan tanaman melon (Cucumis melo L.) terhadap powdery mildew (Podosphaera xanthii (Castag.). Braun et Shishkoff). 15(1), 1-6. https://doi.org/10.22146/jpti.11758. Jurnal Perlindungan Tanaman Indonesia, 15(1), 1–6.
Davis, A. R., Levi, A., Wehner, T., & Pitrat, M. (2006). PI 525088-PMR, a melon race 1 powdery mildew resistant watermelon line. HortScience, 41(7), 1527–1528. Retrieved from https://doi.org/10.21273/HORTSCI.41.7.1527
Eckardt, N. A. (2004). Aminotransferases confer "enzymatic resistance” to downy mildew in melon. Journal of Experimental Botany, 54(384), 1069–1074.
Farr, D. F., & Rossman, A. Y. (2020). Fungal Databases, U.S. National Fungus Collections,. Retrieved March 20, 2020, from ARS, USDA. website: https://nt.ars-grin.gov/fungaldatabases/
Fukino, N., Kunihisa, M., & Matsumoto, S. (2004). Characterization of recombinant inbred lines derived from crosses in melon (Cucumis melo L.), ‘PMAR NO.5' x 'Harukei No.3'. Breeding Science, 54(2), 141–145.
Hong, C., Weiping, K., Junfen, L., & Jiping, L. (2015). Analysis of powdery mildew resistance in wild melon MLO mutans. Horticultural Plant Journal, 1(3), 165–171.
Hong, Y.-J., Hossain, M. R., Kim, H.-T., Park, J.-I., & Nou, I.-S. (2018). Identification of two new races of Podosphaera xanthii causing powdery mildew in melon in South Korea. The Plant Pathology Journal, 34(3), 182–190. Retrieved from https://doi.org/10.5423/PPJ.OA.12.2017.0261
Ishak, M. A., & Daryono, B. S. (2018). Kestabilan karakter fenotip melon (Cucumis melo L. ‘Sun Lady') hasil budidaya di dusun Jamusan, Prambanan, D.I.yogyakarta. Prosiding Seminar Nasional Pendidikan Biologi Dan Saintek III. Surakarta: Universitas Muhammadiyah.
Junior, R. S., Nunes, G. H., Michereff, S. J., Pereira, E. W. L., & Guomaraes, I. M. (2011). Reaction of families and lines of melon to powdery mildew. Horticultura Brasileira, 29(3), 382–386.
Kasiamdari, R. S., Riefani, M. K., & Daryono, B. S. (2016). The occurrence and identification of powdery mildew on melon in Java, Indonesia. AIP Conference Proceeding 1744, 0200050(2016). Retrieved from https://doi.org/10.1063/1.4953524
Marmolejo, J., Siahaan, S. A. S., Takamatsu, S., & Braun, U. (2018). Three new records of powdery mildews found in Mexico with one genus and one new species proposed. Mycoscience, 59(1), 1–7. Retrieved from https://doi.org/10.1016/j.myc.2017.06.010
McCreight, J. D., & Coffey, M. D. (2011). Inheritance of resistance in melon PI 313970 to cucurbit powdery mildew incited by Podosphaera xanthii race s. HortScience, 46(6), 838–840. Retrieved from https://doi.org/10.21273/HORTSCI.46.6.838
Rabelo, H. O., Santos, L. S., Diniz, G. M., Marin, M. V., Braz, L. T., & D., M. J. (2017). Cucurbits powdery mildew race identity and reaction of melon genotypes. Pesq. Agropec. Trop, 47(4), 440–447.
Ramos, C. B., Maruthachalam, K. McCreight, J. D., & Estrada, R. S. G. (2012). Podosphaera xanthii but not Golovinomyces cichoracearum infects cucurbits in a greenhouse at Salinas, California. Cucurbit Genetics Cooperative Report, 33(1), 24–28.
Rivera, M. E., Codina, J. C., Olea, F., de Vicente, A. D., & Perez-Garcia, A. (2002). Differential expression of β-1,3-glucanase in susceptible and resistant melon cultivars in response to infection by Sphaerotheca fusca. Physiological and Molecular Plant Pathology, 61(5), 257–265. Retrieved from https://doi.org/10.1006/pmpp.2002.0436
Stadnik, M. J., Kobori, R. F., & Bettiol, W. (2001). (2001). Oídios de curcubitáceas. In M. J. Stadnik, M. C. Rivera (Ed.), Oídios. Jaguariúna: Embrapa Meio Ambiente.
Surat Keputusan Menteri Pertanian Republik Indonesia, M. (2017). Deskripsi Melon Varietas Meloni. Jakarta: Direktur Jenderal Hortikultura.
Takamatsu, S. (2018). Studies on the evolution and systematics of powdery mildew fungi. Journal of General Plant Pathology, 84, 422–426. Retrieved from https://doi.org/10.1007/s10327-018-0805-4
Vakalounakis, D. J., Klironomou, E., & Papadakis, A. (1994). Species spectrum, host range and distribution of powdery mildews on cucurbitaceae in Crete. Plant Pathology, 43(6), 813–818.
Welsh, J. R. (1991). Dasar-Dasar Genetika dan Pemuliaan Tanaman (J. P. Mogea, Ed.). Jakarta: Erlangga.
Xue-zheng, W., Ping, X. B. W., Fei-shi, L., Hong-yan, M., & Ying-yuan, M. (2013). Identification of powdery mildew pathogen and ribosomal DNA-ITS sequence analysis on melon. Journal of Northeast Agricultural University, 20(4), 10–18.
Aristya, G. Z., Suryanto, E., Kasiamdari, R. S., & Daryono, B. S. (2013). Seleksi Ketahanan Kultivar Tanaman Melon (Cucumis melo L.) Terhadap Jamur Tepung (Powdery Mildew) Isolat Ngawi. Jurnal Penelitian Dan Pengembangan, 8(8), 15–30.
Braun, U., & Cook, R. T. A. (2012). Taxonomic Manual of the Erypshiphales (Powdery Mildews). Utrecht: CBS KNAW fungal Biodiversity Center.
Cho, S. E., Choi, I. Y., Han, K. S., & Shin, H. D. (2017). First record of Podosphaera xanthii on Peperomia tetragona. Australasian Plant Diseases Notes, 12(1), 31.
Crowder, L. V. (1990). Genetika Tumbuhan. Yogyakarta: Gadjah Mada University.
Daryono, B. S., Aristya, G. R., & Kasiamdari, R. S. (2011). Development of random amplified polymorphism DNA markers linked to Powdery Mildew resistance gene in Melon. Indonesian Journal of Biotechnology, 16(2), 76–82. Retrieved from https://doi.org/10.22146/ijbiotech.7837
Daryono, B. S., E., V. A., & Al-Mughni, E. W. (2018). Phenotypic characters stability of melon (Cucumis melo L. Meloni cultivar). The Proceeding of the 2nd International Conference on Tropical Agriculture, 141–149. Yogyakarta.
Daryono, B. S., & Qurrohman, M. T. (2009). Pewarisan sifat ketahanan tanaman melon (Cucumis melo L.) terhadap powdery mildew (Podosphaera xanthii (Castag.). Braun et Shishkoff). 15(1), 1-6. https://doi.org/10.22146/jpti.11758. Jurnal Perlindungan Tanaman Indonesia, 15(1), 1–6.
Davis, A. R., Levi, A., Wehner, T., & Pitrat, M. (2006). PI 525088-PMR, a melon race 1 powdery mildew resistant watermelon line. HortScience, 41(7), 1527–1528. Retrieved from https://doi.org/10.21273/HORTSCI.41.7.1527
Eckardt, N. A. (2004). Aminotransferases confer "enzymatic resistance” to downy mildew in melon. Journal of Experimental Botany, 54(384), 1069–1074.
Farr, D. F., & Rossman, A. Y. (2020). Fungal Databases, U.S. National Fungus Collections,. Retrieved March 20, 2020, from ARS, USDA. website: https://nt.ars-grin.gov/fungaldatabases/
Fukino, N., Kunihisa, M., & Matsumoto, S. (2004). Characterization of recombinant inbred lines derived from crosses in melon (Cucumis melo L.), ‘PMAR NO.5' x 'Harukei No.3'. Breeding Science, 54(2), 141–145.
Hong, C., Weiping, K., Junfen, L., & Jiping, L. (2015). Analysis of powdery mildew resistance in wild melon MLO mutans. Horticultural Plant Journal, 1(3), 165–171.
Hong, Y.-J., Hossain, M. R., Kim, H.-T., Park, J.-I., & Nou, I.-S. (2018). Identification of two new races of Podosphaera xanthii causing powdery mildew in melon in South Korea. The Plant Pathology Journal, 34(3), 182–190. Retrieved from https://doi.org/10.5423/PPJ.OA.12.2017.0261
Ishak, M. A., & Daryono, B. S. (2018). Kestabilan karakter fenotip melon (Cucumis melo L. ‘Sun Lady') hasil budidaya di dusun Jamusan, Prambanan, D.I.yogyakarta. Prosiding Seminar Nasional Pendidikan Biologi Dan Saintek III. Surakarta: Universitas Muhammadiyah.
Junior, R. S., Nunes, G. H., Michereff, S. J., Pereira, E. W. L., & Guomaraes, I. M. (2011). Reaction of families and lines of melon to powdery mildew. Horticultura Brasileira, 29(3), 382–386.
Kasiamdari, R. S., Riefani, M. K., & Daryono, B. S. (2016). The occurrence and identification of powdery mildew on melon in Java, Indonesia. AIP Conference Proceeding 1744, 0200050(2016). Retrieved from https://doi.org/10.1063/1.4953524
Marmolejo, J., Siahaan, S. A. S., Takamatsu, S., & Braun, U. (2018). Three new records of powdery mildews found in Mexico with one genus and one new species proposed. Mycoscience, 59(1), 1–7. Retrieved from https://doi.org/10.1016/j.myc.2017.06.010
McCreight, J. D., & Coffey, M. D. (2011). Inheritance of resistance in melon PI 313970 to cucurbit powdery mildew incited by Podosphaera xanthii race s. HortScience, 46(6), 838–840. Retrieved from https://doi.org/10.21273/HORTSCI.46.6.838
Rabelo, H. O., Santos, L. S., Diniz, G. M., Marin, M. V., Braz, L. T., & D., M. J. (2017). Cucurbits powdery mildew race identity and reaction of melon genotypes. Pesq. Agropec. Trop, 47(4), 440–447.
Ramos, C. B., Maruthachalam, K. McCreight, J. D., & Estrada, R. S. G. (2012). Podosphaera xanthii but not Golovinomyces cichoracearum infects cucurbits in a greenhouse at Salinas, California. Cucurbit Genetics Cooperative Report, 33(1), 24–28.
Rivera, M. E., Codina, J. C., Olea, F., de Vicente, A. D., & Perez-Garcia, A. (2002). Differential expression of β-1,3-glucanase in susceptible and resistant melon cultivars in response to infection by Sphaerotheca fusca. Physiological and Molecular Plant Pathology, 61(5), 257–265. Retrieved from https://doi.org/10.1006/pmpp.2002.0436
Stadnik, M. J., Kobori, R. F., & Bettiol, W. (2001). (2001). Oídios de curcubitáceas. In M. J. Stadnik, M. C. Rivera (Ed.), Oídios. Jaguariúna: Embrapa Meio Ambiente.
Surat Keputusan Menteri Pertanian Republik Indonesia, M. (2017). Deskripsi Melon Varietas Meloni. Jakarta: Direktur Jenderal Hortikultura.
Takamatsu, S. (2018). Studies on the evolution and systematics of powdery mildew fungi. Journal of General Plant Pathology, 84, 422–426. Retrieved from https://doi.org/10.1007/s10327-018-0805-4
Vakalounakis, D. J., Klironomou, E., & Papadakis, A. (1994). Species spectrum, host range and distribution of powdery mildews on cucurbitaceae in Crete. Plant Pathology, 43(6), 813–818.
Welsh, J. R. (1991). Dasar-Dasar Genetika dan Pemuliaan Tanaman (J. P. Mogea, Ed.). Jakarta: Erlangga.
Xue-zheng, W., Ping, X. B. W., Fei-shi, L., Hong-yan, M., & Ying-yuan, M. (2013). Identification of powdery mildew pathogen and ribosomal DNA-ITS sequence analysis on melon. Journal of Northeast Agricultural University, 20(4), 10–18.
Authors
Ishak, M. A., & Daryono, B. S. (2020). Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni. BIOEDUSCIENCE, 4(1), 1–10. https://doi.org/10.29405/j.bes/411-104725
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