Effectiveness of Actinomycetes Isolates from Bogor Botanical Gardens Land as Antifungal against Candida albicans Growth in Vitro
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Submitted
3 February 2020
Published
30 June 2020
Keywords:
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Actinomycetes, Antifungi, Candida albicans, Tanah Actinomycetes, Antifungi, Candida albicans, Soil
Abstract
Background: Actinomycetes are Gram-positive and anaerobic bacteria noted for its rod-shaped forms. Actinomycetes aso has an antibiotic and antifungal activity. This study aims to identify the effectiveness of Actinomycetes isolate as the antifungal toward C. albicans growth by in vitro. Methods: Collected samples were planted on Starch Casein Agar (SCA) through pour plate method with three dilution series of 10-4, 10-5,10-6. The media used to see the obstacles zone of C. albicans was Sabouraud Dextrose Agar (SDA) with well method diffusion. Results: Actinomycetes isolates found in the samples were 15 isolates. From the three-dilution series, the study gets the size average of clear zone 3,78 mm, 4,03 mm and 3,52 mm. One way Anova (α 0,05) result also confirmed the difference of obstacles zone levels between C. albicans concentration. Conclusions: The compound comes from Actinomycetes that has potential as antifungal with one of its mechanism's bonds ergosterol and resists fungi protein synthesis.
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References
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Ambarwati, Azizah, T., Sembiring, L., & Wahyuono, S. (2012). Uji Aktivitas Antifungi Actinomycetes yang Berasosiasi dengan Rizosfer Padi (Oriza sativa), 139–148.
Bahar, M., & Zulfa, F. (2018). Potention of Antibacterial Isolat Actinomycetes to Proteolitic and Amilolitic Activity Escherichia Coli ATTC 25922. Jurnal Teknologi Laboratorium, 7(1), 25. https://doi.org/10.29238/teknolabjournal.v7i1.101
Benhadj, M., Gacemi-Kirane, D., Menasria, T., Guebla, K., & Ahmane, Z. (2018). Screening of rare actinomycetes isolated from natural wetland ecosystem (Fetzara Lake, northeastern Algeria) for hydrolytic enzymes and antimicrobial activities. Journal of King Saud University - Science. https://doi.org/10.1016/j.jksus.2018.03.008
Center for Deasease Control and Prevention (2013). Antibiotik Resistance Threats in the United Stated. US: Department of Health and Human Service.
Davis, W. W., & Stout, T. R. (1971). Disc Plate Method of Microbiological Antibiotic Assay. J.~Chem.~Phys., 84(4), 5970.
Ganesan, P., Reegan, A. D., David, R. H. A., Gandhi, M. R., Paulraj, M. G., Al-Dhabi, N. A., & Ignacimuthu, S. (2017). Antimicrobial activity of some actinomycetes from Western Ghats of Tamil Nadu, India. Alexandria Journal of Medicine, 53(2), 101–110. https://doi.org/10.1016/j.ajme.2016.03.004
Hamidah, Ambarwati, & Peni, I. (2013). Isolasi dan identifikasi isolat actinomycetes dari rizosfer padi (Oryza sativa L.) Sebagai Penghasil Antifungi. Naskah Publikasi, 1–15.
Jones, K. E., Patel, N. G., Levy, M. A., Storeygard, A., Balk, D., Gittleman, J. L., & Daszak, P. (2008). Global trends in emerging infectious diseases. Nature, 451, 990. Retrieved from https://doi.org/10.1038/nature06536
Katzung, B. G. (2018). Basic & Clinical Pharmacology Fourteenth Edition (14th ed.). United States of America: McGraw-Hill Education. https://doi.org/0443069115
Khoirina, A., & Rahayu, T. (2014). Aktivitas Antifungi Isolat Actinomycetes Dari Sampel Gunung Merapi Dengan Lama Fermentasi yang Berbeda Terhadap Candida Albicans. Publikasi Universitas Muhammadiyah Surakarta.
Mohseni, M., Norouzi, H., Hamedi, J., & Roohi, A. (2013). Screening of Antibacterial Producing Actinomycetes from Sediments of the Caspian Sea. Int J Mol Cell Med, 2(2), 64–71. https://doi.org/10.1167/iovs.06-0759
Nurkanto, A., Listyaningsih, F., & Julistiono, H. (2010). Eksplorasi Keanekaragaman Aktinomisetes Tanah Ternate Sebagai Sumber Antibiotik, 6(3), 325–339.
Oskay, M. (2009). Antifungal and antibacterial compounds from Streptomyces strains. African Journal of Biotechnology, 8(13), 3007–3017. https://doi.org/10.1016/j.bjps.2009.06.042
Pfaller, M. A., & Diekema, D. J. (2004). Rare and Emerging Opportunistic Fungal Pathogens: Concern for Resistance beyond. Society, 42(10), 4419–4431. https://doi.org/10.1128/JCM.42.10.4419
Pujiati. (2014). Isolasi Actinomycetes Dari Tanah Kebun Sebagai Bahan Petunjuk Praktikum Mikrobiologi. Jurnal Florea, 1(2), 42–46.
Singh, S., Baruah, I., & Bora, T. . (2006). Actinomycetes of Loktak Habitat: Isolation and Screening for Antimicrobial Activities.
Whaley, S. G., Berkow, E. L., Rybak, J. M., Nishimoto, A. T., Barker, K. S., & Rogers, P. D. (2017). Azole antifungal resistance in Candida albicans and emerging non-albicans Candida Species. Frontiers in Microbiology, 7(JAN), 1–12. https://doi.org/10.3389/fmicb.2016.02173
Yappar, N. (2014). Epidemiology and risk factors for invasive candidiasis. Therapeutics and Clinical Risk Management, 10(1), 95–105. https://doi.org/10.2147/TCRM.S40160
Authors
Aminnullah, R. (2020). Effectiveness of Actinomycetes Isolates from Bogor Botanical Gardens Land as Antifungal against Candida albicans Growth in Vitro. BIOEDUSCIENCE, 4(1), 90–96. https://doi.org/10.29405/j.bes/4190-964362
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