Gel Formulation of Coconut Shell Charcoal Extract and Sensitivity Test for Escherichia coli Bacteria
Article Sidebar
21 June 2023
30 April 2024
Abstract
Background: Escherichia coli bacteria are normal flora bacteria in the human digestive tract that can turn into opportunistic pathogens and cause diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS). The existence of this bacterial infection is generally treated with the use of antibiotics, which have been shown to cause resistance to the E. coli bacteria, so it is necessary to treat them using other methods, namely by using drugs derived from natural ingredients (herbal medicines) that contain antibacterial compounds such as charcoal extract. coconut shell. The purpose of this study was to obtain the most effective coconut shell charcoal extract gel formulation for inhibiting the growth of E. coli bacteria in vitro. Methods: The method used in this study was a true experimental post-test only control group with treatment at different gel concentrations of 3%, 6%, and 9% with repetition. Results: The results of the one-way ANOVA analysis test showed that the treatment given to several concentrations of coconut shell charcoal extract had a mean difference in the area of the inhibition zone for E. coli bacteria with a known significance value of 0.002 (p <0.05), so it was continued with the post-host LSD test, and the results of several groups show a significant value (p <0.05) marked with the notation (*). Conclusion: Based on the results of the study, it can be concluded that the gel formulation of coconut shell charcoal extract with a concentration of 3% is most effective in inhibiting the growth of E. coli bacteria in vitro.
Full text article
References
Dwi, Meircurius & Ira Arundina. (2019). Manfaat Lain Asap Cair (Liquid Smoke) Sebagai Obat Sariawan.
Duma, I., Irianto, K., Mardan, M. T., Farmasi, D. B., Farmasi, F., Mada, U. G., Farmasi, F., & Mada, U. G. (2020). Aktivitas Antibakteri dan Uji Sifat Fisik Sediaan Gel Dekokta Sirih Hijau (Piper betle L.)sSebagai Alternatif Pengobatan Mastitis Sapi Antibacterial Activity and Physical Evaluation of Piper betle L . Decoction Gel as an Alternative Treatment for Masti. Majalah Farmaseutik, 16(2), 202–210. https://doi.org/10.22146/farmaseutik.v16i2.53793
Huda, C., Putri, A. E., & Sari, D. W. (2019). Uji Aktivitas Antibakteri Fraksi Dari Maserat Zibethinus Folium Terhadap Escherichia coli. Jurnal SainHealth, 3(1), 7. https://doi.org/10.51804/jsh.v3i1.333.7-14
Hutasoit, D. (2020). Effect of Food Sanitation and Escherichia coli Bacteria Contamination on Diarrhea. Jurnal Ilmiah Kesehatan Sandi Husada, 9(2), 779-786. https://doi.org/10.35816/jiskh.v12i2.399
KEMENKES RI. (2020). Farmakope Indonesia Edisi VI. Kementerian Kesehatan RI.
Krongrawa, W., Limmatvapirat, S., Pongnimitprasert, N., Meetam, P., & Limmatvapirat, C. (2018). Formulation and Evaluation of Gels Containing Coconut Kernel Extract For Topical Application. Asian Journal of Pharmaceutical Sciences, June, 1–10. https://doi.org/10.1016/j.ajps.2018.01.005
Kurniawan, & Yulistiani, M. (2020). A Production and Activity Test of Antibacterial Compounds of Endophytic Fungi BR-S1 (A) Isolate Extract in Different General Growth Media. Current Trends in Biotechnology and Pharmacy, 14(5), 206–212. https://doi.org/10.5530/ctbp.2020.4s.25
Mazaya, G. (2020). Pengujian Aktivitas Antimikroba, Identifikasi Fitokimia dan Aplikasi Pengawet Alami Nira Berbahan Tempurung Kelapa. Universitas Jendral Sudirman.
Mazaya, G., Karseno, K., & Yanto, T. (2020). Antimicrobial and Phytochemical Activity of Coconut Shell Extracts. Turkish Journal of Agriculture - Food Science and Technology, 8(5), 1090–1097. https://doi.org/10.24925/turjaf.v8i5.1090-1097.3282
Normaliska, R., Sudarwanto, M. B., & Latif, H. (2019). Pola Resistensi Antibiotik pada Escherichia coli Penghasil ESBL dari Sampel Lingkungan di RPH-R Kota Bogor. Acta VETERINARIA Indonesiana, 7(2), 42–48.
Nurjanah, et.al. (2020). Analisis Kandungan Zat Kimia Berbahaya dan Bakteri Patogen pada Jajanan Berbahan Olahan Daging di Sekolah Dasar Negeri Kota Bandung. (IJHS) Indonesian Journal of Halal Science, 001(02).
Philip, P., Kern, D., Goldmanns, J., Seiler, F., Schulte, A., Habicher, T., & Büchs, J. (2018). Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask. Microbial Cell Factories, 17(1), 1–17. https://doi.org/10.1186/s12934-018-0917-8
Prasetya, et.al. (2019). Deteksi Fenotipik Escherichia Coli Penghasil Extended Spectrum Beta-Lactamases (Esbls) Pada Sampel Makanan Di Krian Sidoarjo. Life Science, 8(1).
Prawira, M.Y., Sarwiyono dan P. Surjowardojo. 2013. Daya Hambat Dekok Daun Kersen (Muntingia calabura l.) Terhadap pertumbuhan bakteri S. aureus penyebab penyakit mastitis pada sapi perah.
Pritha SDSJ & Karpagam S. (2019). Antimicrobial activity of coconut shell oil. Int J Pharm Sci Res, 9(4):1628–1631
Purnamasari, R. (2020). Formulasi Sediaan Gel Minyak Kelapa Murni atau VCO ( Virgin Coconut Oil ) yang Digunakan Sebagai Pelembab Wajah. Jurnal Kesehatan Luwu Raya, 6(2), 37–43.
Putri, W. E., & Anindhita, M. A. (2022). Optimization of cardamom fruit ethanol extract gel with combination of HPMC and Sodium Alginate as the gelling agent using Simplex Lattice Design. Jurnal Ilmiah Farmasi (Scientific Journal of Pharmacy) Special Edition, 107–120.
Rizkia, A. D., Syaputri, F. N., & Tugon, T. D. A. (2022). Pengaruh Variasi Konsentrasi Na-CMC sebagai Gelling Agent Terhadap Stabilitas Fisik dan Kimia Sediaan Gel Ekstrak Daun Sereh Wangi (Cymbopogon nardus (L.) Rendle). Jurnal Sains Farmasi, 3(1), 1–11. https://doi.org/10.36456/farmasis.v3i1.5295
Rosyada, A. S., Kurniawan, Mulyanto, A., & Dhanti, K. R. (2023). Pembuatan dan Uji Antibakteri Gel Arang Tempurung Kelapa terhadap Bakteri Staphylococcus aureus. SCRIPTA BIOLOGICA, 10(3), 11–17.
Suhesti, T. S. (2021). Formulation of Gel Hand Sanitizer of Nagasari Leaf Extract (Mesua ferrea L.) Formulasi Sediaan Gel Hand Sanitizer Ekstrak Etanol Daun Nagasari (Mesua ferrea L.). Indonesian Journal of Pharmaceutical Science and Technology, 1(1).
Sutiknowati, L. I. (2016). Bioindikator Pencemar, Bakteri Escherichia coli. Jurnal Oseana, 41(4), 63–71.
Authors
This work is licensed under a Creative Commons Attribution 4.0 International License.
