EVALUASI MUTU PROTEIN BISKUIT YANG MEMANFAATKAN BLONDO DAN DIPERKAYA DENGAN TEPUNG IKAN GABUS

Abstract

ABSTRACT
The objective of this research was to analyze the true digestibility (TD), biological value
(BV), and net protein utilization (NPU) of biscuits. The research used 25 rats of Sprague
dawley aged between 29-32 days. The research was conducted in two stages, adaptation and
intervention period. The period of adaptation was conducted for 4 days by providing the
standard feed (AOAC 1995) and aquades ad libitum. The period of intervention was began by
classifying 25 rats into 5 groups: group A (fed with standard biscuit feed), group B (fed with
blondo biscuit feed), group C (fed with blondo + snakehead biscuit feed), group K1 (fed with
non-protein feed), and group K2 (fed with casein feed). Intervention was carried out for 10
days. During the intervention, a given amount of feed, remaining feed, urine, and feces were
collected and weighed for analysis purposes. Data were analyzed using Analysis of Variants
(ANOVA). If the results of ANOVA showed signifcant differences (p<0,05), then followed by
Duncan's test with 5% confdence interval. The results showed that total of feed intake, weight 

gain, and Feed Conversion Effciency (FCE) were signifcantly different (p<0,05). The value of

the TD, BV, and NPU of casein group, standard biscuit group, blondo biscuit group and blondo
+ snakehead biscuit group were not signifcantly different (p>0,05).