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Background: The transition of the 2013 curriculum to the Merdeka curriculum requires teachers to be more creative, applicable, and able to stimulate students' critical powers in understanding selected subjects. However, students' elective subjects in Modern Biotechnology, such as Plant Tissue Culture, are poorly understood in practice. This activity aims to improve practical skills in plant propagation techniques by providing plant tissue culture techniques for high school/equivalent teachers in the field of Biology in Samarinda and surrounding areas. Method: This service was carried out by Biology lecturers at FMIPA Mulawarman University and was attended by 22 Biology and agriculture teachers in Samarinda and surrounding areas. Service activities consist of 4 stages, namely the initial stage, theoretical provision stage, practicum stage, and evaluation stage. Results: There was an increase in participants' understanding of plant tissue culture from 'poor' to 'good' level. Evaluation of material presentation and suitability of theory and practice reached the very good category with percentages of 80% and 92%, respectively. Reflections on debriefing showed that 80% of participants thought this activity added insight and was fun. Conclusion: Activities were carried out according to the target and can contribute to the student learning process at school, especially in plant tissue culture.
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© 2025 Oleh authors. Lisensi Jurnal SOLMA, LPPM-Uhamka, Jakarta. Artikel ini bersifat open access yang didistribusikan di bawah syarat dan ketentuan Creative Commons Attribution (CC BY) license. (http://creativecommons.org/licenses/by/4.0/).
Referensi
- Alfath, A., Azizah, F. N., & Setiabudi, D. I. (2022). Pengembangan Kompetensi Guru Dalam Menyongsong Kurikulum Merdeka Belajar. Jurnal Riset Sosial Humaniora, Dan Pendidikan, 1(2), 42–50. https://doi.org/10.56444/soshumdik.v1i2.73
- Azizi, A. A. A., Ika, R., & Darda, E. (2017). Multiplikasi Tunas In Vitro Berdasarkan Jenis Eksplan pada Enam Genotipe Tebu (Saccharum officinarum L.). Jurnal Penelitian Tanaman Industri, 23(2), 90–97.
- Dewi, N., Dewi, I. S., & Roostika, I. (2014). Pemanfaatan teknik kultur in vitro untuk konservasi plasma nutfah ubi-ubian. Jurnal AgroBiogen, 10(1), 34–44.
- Karyanti, Kristianto, Y. G., Khairiyah, H., Novita, L., Sukarnih, T., Rudiyana, Y., & Sofia, D. Y. (2018). Pengaruh Wadah Kultur dan Konsentrasi Sumber Karbon Pada Perbanyakan Kentang Atlantik Secara In Vitro. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 5(2), 177. https://doi.org/10.29122/jbbi.v5i2.3012
- Kementerian Pendidikan, Kebudayaan, R. dan T. (2023). Jumlah Data Satuan Pendidikan (Dikmen) Per Prov. Kalimantan Timur.
- Kementerian Pendidikan, K. R. dan T. (2022). Panduan Pemilihan Mata Pelajaran Pilihan. Kurikulum.Kemdikbud.Go.Id, 1–57.
- Kurka. (2022). Struktur Kurikulum Merdeka SMK Pusat Keunggulan.
- Mastuti, R. (2017). Dasar-Dasar Kultur Jaringan Tumbuhan. Malang: UB Press. Brawijaya Press.
- Mirawati, B., Royani, I., Imran, A. M., Firdaus, L., & Fitriyani, H. (2019). Pelatihan Teknik Kultur Jaringan Siswa MA Syaikh Zainuddin (MAPK) NW Anjani Lombok Timur.
- Nurcahyani, D. E., Sumardi, D., Irawan, D. B., Sari, E. Y., & Sari, T. L. (2019). In Vitro Study: Induced Resistance of Cassava (Manihot Esculenta Crantz.) Plantlet Against Fusarium Oxysporum Based on Analysis of Phenol Content.
- Nurcahyani, E., Agustrina, R., Suroso, E., & Andari, G. (2016). Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. International Journal of Applied Agricultural Sciences, 2(6), 79–82. https://doi.org/10.11648/j.ijaas.20160206.11
- Praseptiana, C., Darmanti, S., & Prihastanti, E. (2017). Multiplikasi Tunas Tebu (Saccharumo officinarum L Var. Bululawang) dengan Perlakuan Konsentrasi BAP dan Kinetin Secara In Vitro. Buletin Anatomi Dan Fisiologi, 2(2), 153. https://doi.org/10.14710/baf.2.2.2017.153-160
- Putri, A. B. S., Hajrah, H., Armita, D., & Tambunan, I. R. (2021). Teknik kultur jaringan untuk perbanyakan dan konservasi tanaman kentang (Solanum tuberosum L.) secara in vitro. Filogeni: Jurnal Mahasiswa Biologi, 1(2), 69–76. https://doi.org/10.24252/filogeni.v1i2.23801
- Yacha, A., Sopandi, T., WK, P. S., Binawati, D. K., Ngadiani, Sukarjati, Ajiningrum, P. S., & Andriani, V. (2022). Pengenalan Dan Pelatihan Teknik Kultur Jaringan Tanaman Pada Guru Mgmp Biologi Sma Se-Jawa Timur. Jurnal Penamas Adi Buana, 6(01), 31–36. https://doi.org/10.36456/penamas.vol6.no01.a5498
Referensi
Alfath, A., Azizah, F. N., & Setiabudi, D. I. (2022). Pengembangan Kompetensi Guru Dalam Menyongsong Kurikulum Merdeka Belajar. Jurnal Riset Sosial Humaniora, Dan Pendidikan, 1(2), 42–50. https://doi.org/10.56444/soshumdik.v1i2.73
Azizi, A. A. A., Ika, R., & Darda, E. (2017). Multiplikasi Tunas In Vitro Berdasarkan Jenis Eksplan pada Enam Genotipe Tebu (Saccharum officinarum L.). Jurnal Penelitian Tanaman Industri, 23(2), 90–97.
Dewi, N., Dewi, I. S., & Roostika, I. (2014). Pemanfaatan teknik kultur in vitro untuk konservasi plasma nutfah ubi-ubian. Jurnal AgroBiogen, 10(1), 34–44.
Karyanti, Kristianto, Y. G., Khairiyah, H., Novita, L., Sukarnih, T., Rudiyana, Y., & Sofia, D. Y. (2018). Pengaruh Wadah Kultur dan Konsentrasi Sumber Karbon Pada Perbanyakan Kentang Atlantik Secara In Vitro. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 5(2), 177. https://doi.org/10.29122/jbbi.v5i2.3012
Kementerian Pendidikan, Kebudayaan, R. dan T. (2023). Jumlah Data Satuan Pendidikan (Dikmen) Per Prov. Kalimantan Timur.
Kementerian Pendidikan, K. R. dan T. (2022). Panduan Pemilihan Mata Pelajaran Pilihan. Kurikulum.Kemdikbud.Go.Id, 1–57.
Kurka. (2022). Struktur Kurikulum Merdeka SMK Pusat Keunggulan.
Mastuti, R. (2017). Dasar-Dasar Kultur Jaringan Tumbuhan. Malang: UB Press. Brawijaya Press.
Mirawati, B., Royani, I., Imran, A. M., Firdaus, L., & Fitriyani, H. (2019). Pelatihan Teknik Kultur Jaringan Siswa MA Syaikh Zainuddin (MAPK) NW Anjani Lombok Timur.
Nurcahyani, D. E., Sumardi, D., Irawan, D. B., Sari, E. Y., & Sari, T. L. (2019). In Vitro Study: Induced Resistance of Cassava (Manihot Esculenta Crantz.) Plantlet Against Fusarium Oxysporum Based on Analysis of Phenol Content.
Nurcahyani, E., Agustrina, R., Suroso, E., & Andari, G. (2016). Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. International Journal of Applied Agricultural Sciences, 2(6), 79–82. https://doi.org/10.11648/j.ijaas.20160206.11
Praseptiana, C., Darmanti, S., & Prihastanti, E. (2017). Multiplikasi Tunas Tebu (Saccharumo officinarum L Var. Bululawang) dengan Perlakuan Konsentrasi BAP dan Kinetin Secara In Vitro. Buletin Anatomi Dan Fisiologi, 2(2), 153. https://doi.org/10.14710/baf.2.2.2017.153-160
Putri, A. B. S., Hajrah, H., Armita, D., & Tambunan, I. R. (2021). Teknik kultur jaringan untuk perbanyakan dan konservasi tanaman kentang (Solanum tuberosum L.) secara in vitro. Filogeni: Jurnal Mahasiswa Biologi, 1(2), 69–76. https://doi.org/10.24252/filogeni.v1i2.23801
Yacha, A., Sopandi, T., WK, P. S., Binawati, D. K., Ngadiani, Sukarjati, Ajiningrum, P. S., & Andriani, V. (2022). Pengenalan Dan Pelatihan Teknik Kultur Jaringan Tanaman Pada Guru Mgmp Biologi Sma Se-Jawa Timur. Jurnal Penamas Adi Buana, 6(01), 31–36. https://doi.org/10.36456/penamas.vol6.no01.a5498